Cited by 200Polymer Surface Interaction StudiesAdvanced Cellulose Research StudiesDyeing and Modifying Textile Fibers

Modification of Cellulose Films by Adsorption of CMC and Chitosan for Controlled Attachment of Biomolecules

Orelma, Hannes, Filpponen, Ilari, Johansson, Leena-Sisko, Laine, Janne, Rojas, Orlando J.·2011·Biomacromolecules

Abstract

The adsorption of human immunoglobulin G (hIgG) and bovine serum albumin (BSA) on cellulose supports were investigated. The dynamics and extent of related adsorption processes were monitored by surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D). Amine groups were installed on the cellulose substrate by adsorption of chitosan from aqueous solution, which allowed for hIgG to physisorb from acid media and produced a functionalized substrate with high surface density (10 mg/m(2)). hIgG adsorption from neutral and alkaline conditions was found to yield lower adsorbed amounts. The installation of the carboxyl groups on cellulose substrate via carboxymethylated cellulose (CMC) adsorption from aqueous solution enhanced the physisorption of hIgG at acidic (adsorbed amount of 5.6 mg/m(2)) and neutral conditions. hIgG adsorption from alkaline conditions reduced the surface density. BSA was used to examine protein attachment on cellulose after modification with chitosan or carboxymethyl cellulose. At the isoelectric point of BSA (pI 5), both of the surface modifications enhanced the adsorption of this protein when compared to that on unmodified cellulose (a 2-fold increase from 1.7 to 3.5 mg/m(2)). At pH 4, the electrostatic interactions favored the adsorption of BSA on the CMC-modified cellulose, revealing the affinity of the system and the possibility of tailoring biomolecule binding by choice of the surface modifier and pH of the medium.

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Crossref · 10.1021/bm201236a

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  • Chitosan is the next most abundant natural polysaccharide after cellulose and comprises the exoskeleton of insects and crustaceans and cell walls of fungi.

    “Chitosan ( poly-β-(1→4)-2-amino-2-deoxy-D-glucose), obtained by N-deacetylation (usually over 75%) of chitin, is the next most abundant natural polysaccharide after cellulose (Fig. 6) 59 and comprises the exoskeleton of insects and crustaceans (seafood processing industries' waste products) and cell walls of fungi.60,61”

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Authors
Orelma, H., Filpponen, I., Johansson, L.-S., Laine, J., & Rojas, O. J.
Affiliations
  • Aalto University (FI)
  • North Carolina State University (US)
Corresponding author
  • Filpponen, Ilari
  • Rojas, Orlando J.
Venue
Biomacromolecules
Published
2011-11-15
Volume
12
Issue
12
Pages
4311-4318
ISSN
  • 1525-7797 (ISSN-L)
  • 1526-4602
OpenAlex
W2126901027
Cited by
200
References
60
Citations by year
2026: 9 · 2025: 4 · 2024: 6 · 2023: 7 · 2022: 12 · 2021: 14 · 2020: 13 · 2019: 15 · 2018: 12 · 2017: 20 · 2016: 18 · 2015: 16 · 2014: 14 · 2013: 24 · 2012: 16
FWCI
7.97
Type
journal article
Language
en
Open access
closed
Locations
  • Biomacromolecules (publishedVersion)
  • PubMed (publishedVersion)
Funders
Tekes
Topics
  • Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
  • Physical Sciences › Materials Science › Biomaterials › Advanced Cellulose Research Studies
  • Physical Sciences › Engineering › Building and Construction › Dyeing and Modifying Textile Fibers
Keywords
cellulose films, chitosan adsorption, carboxymethyl cellulose, human immunoglobulin G, bovine serum albumin, protein adsorption, surface functionalization, amino groups, carboxyl groups, pH effect on adsorption, electrostatic interactions, isoelectric point, biomolecule attachment, surface plasmon resonance, QCM-D
MeSH terms
Adsorption, Amines, Cellulase, Cellulose, Immunoglobulin G, Protein Binding, Serum Albumin, Bovine, Surface Properties, Surface Plasmon Resonance, Chitosan, Static Electricity, Quartz Crystal Microbalance Techniques

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