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Mechanism of Cellular Uptake of Highly Fluorescent Conjugated Polymer Nanoparticles
Open accessAbstract
Conjugated polymer nanoparticles are formed by precipitation of highly fluorescent conjugated polymers to form small nanoparticles with extremely bright fluorescence. We characterized cellular uptake and cytotoxicity of 18 ± 5 nm PFBT conjugated polymer nanoparticles in J774A.1 cells. Significant nanoparticle uptake was observed, indicating efficient nanoparticle entry into cells, even for short (1 h) incubations. The high fluorescence of these nanoparticles allows extremely low loading concentrations; PFBT nanoparticle fluorescence in cells could be detected with loading concentrations of 155 pM (270 ppb). Cellular uptake slows at low temperature, consistent with endocytic entry. Nanoparticles colocalize with Texas Red dextran and are trafficked to lysosomes, as demonstrated by the location of nanoparticle fluorescence in perinuclear organelles that also stain with an anti-LAMP-1 antibody. Inhibition of uptake by phosphoinositide 3-kinase inhibitors implicates macropinocytosis as the operative endocytic mechanism. No significant cytotoxic or inflammatory effects could be observed, making PFBT nanoparticles attractive probes for live cell imaging.
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Doherty, G. J., & McMahon, H. T. (2009) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.78.081307.110540 · Cited by 3,075
Immunological properties of engineered nanomaterials
Dobrovolskaia, M. A., & McNeil, S. E. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.223 · Cited by 1,823
Araki, N., Johnson, M. T., & Swanson, J. A. (1996) · The Journal of cell biology · doi:10.1083/jcb.135.5.1249 · Cited by 949
Ryman-Rasmussen, J. P., Riviere, J. E., & Monteiro-Riviere, N. A. (2007) · Journal of Investigative Dermatology · doi:10.1038/sj.jid.5700508 · Cited by 357
Cited by
Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine
Sousa de Almeida, M., Susnik, E., Drasler, B., Taladriz-Blanco, P., Petri-Fink, A., & Rothen-Rutishauser, B. (2021) · Chemical Society Reviews · doi:10.1039/d0cs01127d · Cited by 1,079
Duan, X., Xiao, J., Yin, Q., Zhang, Z., Yu, H., Mao, S., & Li, Y. (2013) · ACS Nano · doi:10.1021/nn4010796 · Cited by 379
Mao, A. S., Shin, J.-W., Utech, S., Wang, H., Uzun, O., Li, W., Cooper, M., Hu, Y., Zhang, L., Weitz, D. A., et al. (2017) · Nature Materials · doi:10.1038/nmat4781 · Cited by 371
Design and development of fluorescent nanostructures for bioimaging
Chen, M., & Yin, M. (2014) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2013.11.001 · Cited by 289
Multicolor Fluorescent Semiconducting Polymer Dots with Narrow Emissions and High Brightness
Rong, Y., Wu, C., Yu, J., Zhang, X., Ye, F., Zeigler, M., Gallina, M. E., Wu, I.-C., Zhang, Y., Chan, Y.-H., et al. (2013) · ACS Nano · doi:10.1021/nn304376z · Cited by 215
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Metadata
- Authors
- Fernando, L. P., Kandel, P. K., Yu, J., McNeill, J., Ackroyd, P. C., & Christensen, K. A.
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-09-23
- Volume
- 11
- Issue
- 10
- Pages
- 2675-2682
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2027548179
- Cited by
- 196
- References
- 4
- FWCI
- 6.10
- Type
- journal article
- Language
- en
- Open access
- green
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