The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery
Open accessAbstract
Existing techniques to encapsulate cells into microscale hydrogels generally yield high polymer-to-cell ratios and lack control over the hydrogel’s mechanical properties1. Here, we report a microfluidic-based method for encapsulating single cells in a ~6 micron layer of alginate that increases the proportion of cell-containing microgels by 10-fold, with encapsulation efficiencies over 90%. We show that in vitro cell viability was maintained over a three-day period, that the microgels are mechanically tractable, and that for microscale cell assemblages of encapsulated marrow stromal cells cultured in microwells, osteogenic differentiation of encapsulated cells depends on gel stiffness and cell density. We also show that intravenous injection of singly-encapsulated marrow stromal cells into mice delays clearance kinetics and sustains donor-derived soluble factors in vivo. The encapsulation of single cells in tunable hydrogels should find use in a variety of tissue engineering and regenerative medicine applications.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Alginate: Properties and biomedical applications
Lee, K. Y., & Mooney, D. J. (2012) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2011.06.003 · Cited by 8,095
Self-citationAlginate hydrogels as synthetic extracellular matrix materials
Rowley, J. A., Madlambayan, G., & Mooney, D. J. (1999) · Biomaterials · doi:10.1016/s0142-9612(98)00107-0 · Cited by 2,342
Self-citationHarnessing traction-mediated manipulation of the cell/matrix interface to control stem-cell fate
Huebsch, N., Arany, P. R., Mao, A. S., Shvartsman, D., Ali, O. A., Bencherif, S. A., Rivera-Feliciano, J., & Mooney, D. J. (2010) · Nature Materials · doi:10.1038/nmat2732 · Cited by 1,569
Self-citationFischer, U. M., Harting, M. T., Jimenez, F., Monzon-Posadas, W. O., Xue, H., Savitz, S. I., Laine, G. A., & Cox, C. S. (2009) · Stem Cells and Development · doi:10.1089/scd.2008.0253 · Cited by 1,292
Substrate stress relaxation regulates cell spreading
Chaudhuri, O., Gu, L., Darnell, M., Klumpers, D., Bencherif, S. A., Weaver, J. C., Huebsch, N., & Mooney, D. J. (2015) · Nature Communications · doi:10.1038/ncomms7365 · Cited by 889
Self-citationGeneration of Lymphohematopoietic Cells from Embryonic Stem Cells in Culture
Nakano, T., Kodama, H., & Honjo, T. (1994) · Science · doi:10.1126/science.8066449 · Cited by 871
Chaudhuri, O., Koshy, S. T., Branco da Cunha, C., Shin, J.-W., Verbeke, C. S., Allison, K. H., & Mooney, D. J. (2014) · Nature Materials · doi:10.1038/nmat4009 · Cited by 820
Self-citationStem Cells in the Treatment of Refractory Chronic Migraines
Mauskop, A., & Rothaus, K. O. (2017) · Case Reports in Neurology · doi:10.1159/000477393 · Cited by 793
Biocompatible surfactants for water-in-fluorocarbon emulsions
Holtze, C., Rowat, A. C., Agresti, J. J., Hutchison, J. B., Angilè, F. E., Schmitz, C. H. J., Köster, S., Duan, H., Humphry, K. J., Scanga, R. A., et al. (2008) · Lab on a Chip · doi:10.1039/b806706f · Cited by 670
Self-citationMonodisperse Alginate Hydrogel Microbeads for Cell Encapsulation
Tan, W. ‐., & Takeuchi, S. (2007) · Advanced Materials · doi:10.1002/adma.200700433 · Cited by 599
Cited by
Mechanical forces direct stem cell behaviour in development and regeneration
Vining, K. H., & Mooney, D. J. (2017) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.108 · Cited by 1,656
Self-citationHydrogel microparticles for biomedical applications
Daly, A. C., Riley, L., Segura, T., & Burdick, J. A. (2019) · Nature Reviews Materials · doi:10.1038/s41578-019-0148-6 · Cited by 1,249
Functional and Biomimetic Materials for Engineering of the Three-Dimensional Cell Microenvironment
Huang, G., Li, F., Zhao, X., Ma, Y., Li, Y., Lin, M., Jin, G., Lu, T. J., Genin, G. M., & Xu, F. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00094 · Cited by 825
Recent Advances in Droplet Microfluidics
Ding, Y., Howes, P. D., & deMello, A. J. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b05047 · Cited by 357
Rational Design of Biomaterials to Potentiate Cancer Thermal Therapy
Zhu, Y., Li, Q., Wang, C., Hao, Y., Yang, N., Chen, M., Ji, J., Feng, L., & Liu, Z. (2023) · Chemical Reviews · doi:10.1021/acs.chemrev.2c00822 · Cited by 163
Metadata
- Authors
- Mao, A. S., Shin, J.-W., Utech, S., Wang, H., Uzun, O., Li, W., Cooper, M., Hu, Y., Zhang, L., Weitz, D. A., & Mooney, D. J.
- Venue
- Nature Materials
- Published
- 2016-10-31
- Volume
- 16
- Issue
- 2
- Pages
- 236-243
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2539835785
- Cited by
- 371
- References
- 28
- FWCI
- 16.31
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery. https://app.cochat.ai/papers/10.1038/nmat4781
- The paper
- Mao, A. S., Shin, J.-W., Utech, S., Wang, H., Uzun, O., Li, W., Cooper, M., Hu, Y., Zhang, L., Weitz, D. A., & Mooney, D. J. (2017). Deterministic encapsulation of single cells in thin tunable microgels for niche modelling and therapeutic delivery. Nature Materials. https://doi.org/10.1038/nmat4781
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
