Design and biophysical characterization of bioresponsive degradable poly(dimethylaminoethyl methacrylate) based polymers for in vitro dna transfection
Abstract
Water-soluble, degradable polymers based on poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA) with low cytotoxicity and good p-DNA transfection efficiency are highlighted in this article. To solve the nondegradability issue of PDMAEMA, new polymers based on DMAEMA and 5,6-benzo-2-methylene-1,3-dioxepane (BMDO) for gene transfection were synthesized. A poly(ethylene oxide) (PEO) azo-initiator was used as free-radical initiator. PEGylation was performed to improve water solubility and to reduce cytotoxicity of the polymers. The resulting polymers contain hydrolyzable ester linkages in the backbone and were soluble in water even with very high amounts of ester linkages. These degradable copolymers showed significantly less toxicity with a MTT assay using L929 cell lines and demonstrated promising DNA transfection efficiency when compared with the gold standard poly(ethyleneimine). Bioresponsive properties of the corresponding quaternized DMAEMA based degradable polymers were also studied. Although the quaternized DMAEMA copolymers showed enhanced water solubility, they were inferior in gene transfection and toxicity as compared to the unquaternized copolymers.
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Increasing interest in biodegradable polyesters and the versatility of RROP with respect to structures accessible via different monomers and their copolymerization with conventional vinyl monomers has changed the situation significantly.
“However, with the increasing interest in biodegradable polyesters and the versatility of RROP with respect to the structures accessible via very different monomers [25] and their copolymerization with conventional vinyl monomers [26 -34] has changed the situation significantly.”
From Ring-Opening Polymerization—An Introductory Review (2013) · cites this paper as [31]
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“However, with the increasing interest in biodegradable polyesters and the versatility of RROP with respect to the structures accessible via very different monomers [25] and their copolymerization with conventional vinyl monomers [26–34] has changed the situation significantly.”
- Authors
- Zhang, Y., Zheng, M., Kissel, T., & Agarwal, S.
- Affiliations
- Philipps University of Marburg (DE)
- Corresponding author
- Kissel, Thomas
- Agarwal, Seema
- ORCID
- Agarwal, Seema
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2012-01-17
- Volume
- 13
- Issue
- 2
- Pages
- 313-322
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2064468792
- Cited by
- 60
- References
- 33
- Citations by year
- 2026: 4 · 2024: 5 · 2023: 4 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 1 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 11 · 2012: 3
- FWCI
- 2.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomacromolecules (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Chemistry › Organic Chemistry › Advanced Polymer Synthesis and Characterization
- Physical Sciences › Materials Science › Polymers and Plastics › Dendrimers and Hyperbranched Polymers
- Keywords
- PDMAEMA, degradable polymers, transfection, DNA transfection, biodegradable copolymers, PEGylation, cytotoxicity, water solubility, quaternization, L929 fibroblasts
- MeSH terms
- Animals, Biocompatible Materials, Cell Survival, DNA, Methacrylates, Magnetic Resonance Spectroscopy, Nylons, Particle Size, Polyethylene Glycols, Polyethyleneimine, Solubility, Transfection, Water, Gene Transfer Techniques, Microscopy, Confocal, Cell Line, Tumor, Mice
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