Fabrication and Characterization of a Nitric Oxide-Releasing Nanofibrous Gelatin Matrix
Abstract
Nitric oxide (NO) plays an important role in cardiovascular homeostasis, immune responses, and wound repair. The pro-angiogenic and antimicrobial properties of NO has stimulated the development of NO-releasing materials for wound dressings. Gelatin, an abundant natural biodegradable polymer derived from collagen, is able to promote wound repair. S-Nitroso-N-acetylpenicillamine (SNAP) can release NO under physiological conditions and when exposed to light. The objective of this project was to fabricate a NO-releasing gelatin-based nanofibrous matrix with precise light-controllable ability. Results showed that under controlled phase separation fabrication conditions, the gelatin formed a highly porous matrix with the nanofiber diameter ranging from 50 to 500 nm. Importantly, the removal of the trace amount of divalent metal ions within gelatin generated a more stable nanofibrous structure. N-acetyl-D-penicillamine (NAP) was functionalized onto the matrix and nitrosated with t-butyl nitrite, yielding a SNAP-gelatin matrix. Analysis of the photoinitiated NO-release showed that the SNAP-gelatin matrices released NO in a highly controllable manner. Application of increasing light intensities yielded increased NO flux from the matrices. In addition, the dried matrices stored in dark at 4 °C maintained stable NO storage capacity, and the purified (ion-removed) gelatin preserved higher NO-releasing capacity than nonpurified gelatin. The antibacterial effect from the SNAP-gelatin matrices was demonstrated by exposing Staphylococcus aureus ( S. aureus ) to a light-triggered NO flux. This controllable NO-releasing scaffold provides a potential antibacterial therapeutic approach to combat drug resistant bacteria.
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Frost et al. developed a SNAP functionalized polymer matrix that can release NO via light-initiated SNAP decomposition.
“Frost et al. successfully develop a S-nitroso-N-acetyl-D-penicillamine functionalized polymer matrix (e.g., PDMS,226 cyclam243 and gelatin222) that can release NO via light-initiated SNAP decomposition.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [222]
SNAP/gelatin antibacterial activity against S. aureus (3 × 105 CFU/mL) created zones of inhibition of 1.2 ± 0.7 mm and 0.75 ± 0.3 mm at 4.5 V and 3 V LED light over 24 h.
“The antibacterial activity of SNAP/gelatin was tested against S. aureus (3 × 105 CFU/mL) and it demonstrated the ability to create a zone of inhibition of 1.2 ± 0.7 mm and 0.75 ± 0.3 mm, when exposed to the 4.5 V and 3 V LED light, respectively, over 24 h.”
From Recent advances in thromboresistant and antimicrobial polymers for biomedical applications: just say yes to nitric oxide (NO) (2016) · cites this paper as [222]
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Frost, M. C., Reynolds, M. M., & Meyerhoff, M. E. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2004.06.006 · Cited by 343 · see citing works
Self-citationComparative stability studies of poly(2-methyl-2-oxazoline) and poly(ethylene glycol) brush coatings
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Dynamic state of S-nitrosothiols in human plasma and whole blood
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Self-citationAthanasopoulos, A. N. (2006) · Blood · doi:10.1182/blood-2005-08-3140 · Cited by 109 · see citing works
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Wo, Y., Brisbois, E. J., Bartlett, R. H., & Meyerhoff, M. E. (2016) · Biomaterials Science · doi:10.1039/c6bm00271d · Cited by 251 · see citing works
- Authors
- Vogt, C., Xing, Q., He, W., Li, B., Frost, M. C., & Zhao, F.
- Affiliations
- Michigan Technological University (US)
- Corresponding author
- Zhao, Feng
- Venue
- Biomacromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-07-29
- Volume
- 14
- Issue
- 8
- Pages
- 2521-2530
- ISSN
- 1525-7797 (ISSN-L)
- 1526-4602
- OpenAlex
- W2014649832
- Cited by
- 45
- References
- 5
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 6 · 2018: 5 · 2017: 1 · 2016: 4 · 2015: 3 · 2014: 2 · 2013: 1
- FWCI
- 1.27
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biomacromolecules (publishedVersion)
- PubMed (publishedVersion)
- Digital Commons - Michigan Tech (Michigan Technological University) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- nitric oxide release, gelatin, S-nitroso-N-acetylpenicillamine, light-triggered drug release, wound dressings, Staphylococcus aureus, divalent metal ions, phase separation, nitrosation, drug-resistant bacteria
- MeSH terms
- Anti-Bacterial Agents, Bandages, Calcium, Copper, Drug Stability, Gelatin, Iron, Light, Microbial Sensitivity Tests, Staphylococcus aureus, Porosity, Spectroscopy, Fourier Transform Infrared, Nitric Oxide Donors, S-Nitroso-N-Acetylpenicillamine, Photochemical Processes, Nanofibers
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