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Measuring Picomolar Intracellular Exchangeable Zinc in PC-12 Cells Using a Ratiometric Fluorescence Biosensor
Abstract
Zinc plays both physiological and pathological roles in biology, making it of increasing interest. To date, intracellular free zinc has been measured in cell types supplemented with or enriched in zinc, such as hippocampal neurons. Here we quantitatively image intracellular exchangeable zinc in an ordinary resting cell culture line (PC-12), using an excitation ratiometric fluorescent biosensor based on carbonic anhydrase (CA). Human CA II has a K d of 4 pM for zinc and suffers no interference from millimolar calcium or magnesium ions. The CA-based biosensor was readily introduced into the cell by a novel approach: fusing a transactivator of transcription (TAT)-derived cell penetrating peptide to the CA molecule and adding it to the cells. Our results indicate that the resting concentration is approximately 5-10 pM in cytoplasm and nucleus. Interestingly, the tetrakis(2-pyridylmethyl)ethylenediamine (TPEN)-Zn complex and TPEN are both apoptogenic for this cell line.
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Cites
Jiang, D., Sullivan, P. G., Sensi, S. L., Steward, O., & Weiss, J. H. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m108834200 · Cited by 265
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Krężel, A., & Maret, W. (2006) · JBIC Journal of Biological Inorganic Chemistry · doi:10.1007/s00775-006-0150-5 · Cited by 406
Metalloproteomics, metalloproteomes, and the annotation of metalloproteins
Maret, W. (2010) · Metallomics · doi:10.1039/b915804a · Cited by 172
Zinc ions modulate protein tyrosine phosphatase 1B activity
Bellomo, E., Massarotti, A., Hogstrand, C., & Maret, W. (2014) · Metallomics · doi:10.1039/c4mt00086b · Cited by 108
Metal site occupancy and allosteric switching in bacterial metal sensor proteins
Guerra, A. J., & Giedroc, D. P. (2012) · Archives of Biochemistry and Biophysics · doi:10.1016/j.abb.2011.11.021 · Cited by 77
Metadata
- Authors
- Bozym, R. A., Thompson, R. B., Stoddard, A. K., & Fierke, C. A.
- Venue
- ACS Chemical Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-03-01
- Volume
- 1
- Issue
- 2
- Pages
- 103-111
- ISSN
- 1554-8929 (ISSN-L)
- 1554-8937
- OpenAlex
- W2008891372
- Cited by
- 240
- References
- 1
- FWCI
- 8.58
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bozym, R. A., Thompson, R. B., Stoddard, A. K., & Fierke, C. A. (2006). Measuring Picomolar Intracellular Exchangeable Zinc in PC-12 Cells Using a Ratiometric Fluorescence Biosensor. ACS Chemical Biology. https://doi.org/10.1021/cb500043a
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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