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Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer
Abstract
Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1-5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K(d)) of 4.95 +/- 0.31 and 7.05 +/- 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 microg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.
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Cites
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Zuker, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg595 · Cited by 13,404
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Bissen, M., & Frimmel, F. H. (2003) · Acta hydrochimica et hydrobiologica · doi:10.1002/aheh.200390025 · Cited by 916
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Remediation technologies for heavy metal contaminated groundwater
Hashim, M. A., Mukhopadhyay, S., Sahu, J. N., & Sengupta, B. (2011) · Journal of Environmental Management · doi:10.1016/j.jenvman.2011.06.009 · Cited by 961
Shen, S., Li, X.-F., Cullen, W. R., Weinfeld, M., & Le, X. C. (2013) · Chemical Reviews · doi:10.1021/cr300015c · Cited by 818
Dave, N., Chan, M. Y., Huang, P.-J. J., Smith, B. D., & Liu, J. (2010) · Journal of the American Chemical Society · doi:10.1021/ja106098j · Cited by 461
Metadata
- Authors
- Kim, M., Um, H.-J., Bang, S., Lee, S.-H., Oh, S.-J., Han, J.-H., Kim, K.-W., Min, J., & Kim, Y.-H.
- Venue
- Environmental Science & Technology
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-11-12
- Volume
- 43
- Issue
- 24
- Pages
- 9335-9340
- ISSN
- 0013-936X (ISSN-L)
- 1520-5851
- OpenAlex
- W2082540323
- Cited by
- 174
- References
- 2
- FWCI
- 3.22
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kim, M., Um, H.-J., Bang, S., Lee, S.-H., Oh, S.-J., Han, J.-H., Kim, K.-W., Min, J., & Kim, Y.-H. (2009). Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer. Environmental Science & Technology. https://doi.org/10.1021/es902407g
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