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Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer

Kim, Mina, Um, Hyun-Ju, Bang, SunBaek, Lee, Sang-Hee, Oh, Suk-Jung, Han, Ji-Hye, Kim, Kyoung-Woong, Min, Jiho, Kim, Yang-Hoon·2009·Environmental Science & Technology

Abstract

Single-stranded DNA aptamers were generated from a random library to remove arsenic from Vietnamese groundwater. On the basis of significant arsenic contamination levels, three areas in Ha Nam province (Vinh Tru, Bo De, and Hoa Hau) and five areas near the Mekong River Delta (MR1-5) were selected as study areas. The aptamers were in vitro selected using an arsenic aptamer affinity column created by immobilizing arsenic on Affi-gel 10 resin. Quantitative analyses of the aptamer candidates Ars-1 to Ars-8 by surface plasmon resonance (SPR) revealed the Ars-3 aptamer to have the highest affinity to arsenate [(As(V)] and arsenite [As(III)] with a dissociation constant (K(d)) of 4.95 +/- 0.31 and 7.05 +/- 0.91 nM, respectively. The specific affinity interactions of the Ars-3 aptamer to arsenic were verified against other heavy metals. After obtaining successful removal results with a laboratory-prepared aqueous arsenic solution, Ars-3 was applied for removal of any arsenic present in the groundwater samples collected from the studied areas in Vietnam. Field results were also successful: various arsenic concentrations ranging from 28.1 to 739.2 microg/L were completely removed after 5 min of incubation with the arsenic-binding aptamer Ars-3.

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Metadata

Authors
Kim, M., Um, H.-J., Bang, S., Lee, S.-H., Oh, S.-J., Han, J.-H., Kim, K.-W., Min, J., & Kim, Y.-H.
Venue
Environmental Science & Technology
Published
2009-11-12
Volume
43
Issue
24
Pages
9335-9340
ISSN
  • 0013-936X (ISSN-L)
  • 1520-5851
OpenAlex
W2082540323
Cited by
174
References
2
FWCI
3.22
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer. https://app.cochat.ai/papers/10.1021/es902407g
The paper
Kim, M., Um, H.-J., Bang, S., Lee, S.-H., Oh, S.-J., Han, J.-H., Kim, K.-W., Min, J., & Kim, Y.-H. (2009). Arsenic Removal from Vietnamese Groundwater Using the Arsenic-Binding DNA Aptamer. Environmental Science & Technology. https://doi.org/10.1021/es902407g

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