The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Diheteroarylethenes as Thermally Stable Photoswitchable Acceptors in Photochromic Fluorescence Resonance Energy Transfer (pcFRET)
Abstract
We have employed diheteroarylethenes as acceptors for photochromic FRET (pcFRET), a technique introduced for the quantitative determination of fluorescence resonance energy transfer (FRET). In pcFRET, the fluorescent emission of the donor is modulated by cyclical transformations of a photochromic acceptor. Light induces a reversible change in the structure and, concomitantly, in the absorption properties of the acceptor. Only the closed forms of the selected diheteroarylethenes 2a and 2b have an absorption band overlapping the emission band of the donor, 1. The corresponding variation in the overlap integral (and thus critical transfer distance R(o)) between the two states provides the means for reversibly switching the process of FRET on and off, allowing direct and repeated evaluation of the relative changes in the donor fluorescence quantum yield. The diheteroarylethenes demonstrate excellent stability in aqueous media, an absence of thermal back reactions, and negligible fatigue. The equilibration of these systems after exposure to near-UV or visible light follows simple monoexponential kinetics. We developed a general conceptual scheme for such coupled photochromic-FRET reactions, allowing quantitative interpretations of the photostationary and kinetic data, from which the quantum yields for the cyclization and cycloreversion reactions of the photochromic acceptor were calculated.
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Cited by
Photochromism of Diarylethene Molecules and Crystals: Memories, Switches, and Actuators
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Self-citationFluorescent Proteins and Their Applications in Imaging Living Cells and Tissues
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Photoactivatable fluorescent proteins
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Reversibly Photoswitchable Dual-Color Fluorescent Nanoparticles as New Tools for Live-Cell Imaging
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Metadata
- Authors
- Giordano, L., Jovin, T. M., Irie, M., & Jares-Erijman, E. A.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-06-01
- Volume
- 124
- Issue
- 25
- Pages
- 7481-7489
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2008351625
- Cited by
- 400
- References
- 19
- FWCI
- 10.76
- Type
- journal article
- Language
- en
- Open access
- closed
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