Cited by 932Advanced Fluorescence Microscopy TechniquesPhotochemistry and Electron Transfer StudiesMolecular Sensors and Ion Detection

Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

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Sekar, Rajesh Babu, Periasamy, Ammasi·2003·The Journal of Cell Biology

Abstract

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.

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Crossref · 10.1083/jcb.200210140

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  1. Herman, B. 1998. Fluorescence Microscopy. 2nd ed. Springer-Verlag New York Inc., New York. 170 pp.

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Authors
Sekar, R. B., & Periasamy, A.
Affiliations
University of Virginia (US)
Corresponding author
Periasamy, Ammasi
Venue
The Journal of Cell Biology
Published
2003-03-03
Volume
160
Issue
5
Pages
629-633
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W2102557954
Cited by
932
References
29
Citations by year
2026: 32 · 2025: 42 · 2024: 71 · 2023: 58 · 2022: 52 · 2021: 55 · 2020: 52 · 2019: 49 · 2018: 51 · 2017: 33 · 2016: 36 · 2015: 39 · 2014: 34 · 2013: 32 · 2012: 21
FWCI
22.00
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
Keywords
fluorescence resonance energy transfer, live-cell imaging, protein localization, protein-protein interaction, fluorescent probes, super-resolution microscopy
MeSH terms
Animals, Eukaryotic Cells, Fluorescent Dyes, Humans, Image Processing, Computer-Assisted, Luminescent Proteins, Microscopy, Fluorescence, Proteins, Fluorescence Resonance Energy Transfer, Green Fluorescent Proteins

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