Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations
Open accessAbstract
The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,948 · see citing works
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Miyawaki, A., Llopis, J. L., Heim, R., McCaffery, J. M., Adams, J. A., Ikura, M., & Tsien, R. Y. (1997) · Nature · doi:10.1038/42264 · Cited by 3,250 · see citing works
Szabó, A. (1982) · Cited by 2,620 · see citing works
Heim, R., & Tsien, R. Y. (1996) · Current Biology · doi:10.1016/s0960-9822(02)00450-5 · Cited by 1,490 · see citing works
Use of resonance energy transfer to monitor membrane fusion
Struck, D. K., Hoekstra, D., & Pagano, R. E. (1981) · Biochemistry · doi:10.1021/bi00517a023 · Cited by 1,281 · see citing works
Fluorescence resonance energy transfer
Clegg, R. M. (1995) · Current Opinion in Biotechnology · doi:10.1016/0958-1669(95)80016-6 · Cited by 932 · see citing works
Quantitative Fluorescence Resonance Energy Transfer Measurements Using Fluorescence Microscopy
Gordon, G. W., Berry, G., Liang, X. H., Levine, B., & Herman, B. A. (1998) · Biophysical Journal · doi:10.1016/s0006-3495(98)77976-7 · Cited by 856 · see citing works
Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell
Bastiaens, P. I. H., & Bastiaens, P. I. H. (1999) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01410-x · Cited by 719 · see citing works
Fluorescence ratio imaging of cyclic AMP in single cells
Adams, S., Harootunian, A. T., Buechler, Y. J., Taylor, S. S., & Tsien, R. Y. (1991) · Nature · doi:10.1038/349694a0 · Cited by 696 · see citing works
Localized Rac Activation Dynamics Visualized in Living Cells
Kraynov, V. S., Chamberlain, C. E., Bokoch, G., Schwartz, M. A., Slabaugh, S., & Hahn, K. M. (2000) · Science · doi:10.1126/science.290.5490.333 · Cited by 678 · see citing works
Xia, Z., & Liu, Y. (2001) · Biophysical Journal · doi:10.1016/s0006-3495(01)75886-9 · Cited by 520 · see citing works
Diffusional Mobility of Golgi Proteins in Membranes of Living Cells
Cole, N. B., Smith, C. L., Sciaky, N., Terasaki, M., Edidin, M. A., & Lippincott‐Schwartz, J. (1996) · Science · doi:10.1126/science.273.5276.797 · Cited by 517 · see citing works
Using GFP in FRET-based applications
Pollok, B. A. (1999) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01434-2 · Cited by 482 · see citing works
Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells
Ting, A. Y., Kain, K. H., Klemke, R. L., & Tsien, R. Y. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.211564598 · Cited by 460 · see citing works
Gadella, T. W. J., & Jovin, T. M. (1995) · The Journal of Cell Biology · doi:10.1083/jcb.129.6.1543 · Cited by 438 · see citing works
Giordano, L., Jovin, T. M., Irie, M., et al. (2002) · Journal of the American Chemical Society · doi:10.1021/ja016969k · Cited by 400 · see citing works
Truong, K., & Ikura, M. (2001) · Current Opinion in Structural Biology · doi:10.1016/s0959-440x(00)00249-9 · Cited by 356 · see citing works
Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI
del Pozo, M. Á., Kiosses, W. B., Alderson, N. B., Meller, N., Hahn, K. M., & Schwartz, M. A. (2002) · Nature Cell Biology · doi:10.1038/ncb759 · Cited by 326 · see citing works
Fluorescence imaging spectroscopy and microscopy
(1997) · Choice Reviews Online · doi:10.5860/choice.34-2759 · Cited by 304 · see citing works
van Roessel, P., & Brand, A. H. (2002) · Nature Cell Biology · doi:10.1038/ncb0102-e15 · Cited by 234 · see citing works
Elangovan, M., Day, R. N., & Periasamy, A. (2002) · Journal of Microscopy · doi:10.1046/j.0022-2720.2001.00984.x · Cited by 233 · see citing works
Self-citationCharacterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy
Elangovan, M., Wallrabe, H. K., Chen, Y., Day, R. N., Barroso, M., & Periasamy, A. (2003) · Methods · doi:10.1016/s1046-2023(02)00283-9 · Cited by 229 · see citing works
Self-citation(2001) · Springer eBooks · doi:10.1007/978-1-4614-7513-2 · Cited by 208 · see citing works
FRET-based in vivo Ca2+ imaging by a new calmodulin-GFP fusion molecule.
Truong, K., Sakaue-Sawano, A., Mizuno, H., Hama, H., Tong, K. I., Mal, T. K., Miyawaki, A., & Ikura, M. (2001) · Nature Structural Biology · doi:10.1038/nsb728 · Cited by 202 · see citing works
GFP imaging: methodology and application to investigate cellular compartmentation in plants
Hanson, M. R., & Köhler, R. H. (2001) · Journal of Experimental Botany · doi:10.1093/jxb/52.356.529 · Cited by 180 · see citing works
GFP imaging: methodology and application to investigate cellular compartmentation in plants
Hanson, M. R., & Köhler, R. H. (2001) · Journal of Experimental Botany · doi:10.1093/jexbot/52.356.529 · Cited by 178 · see citing works
Fluorescence energy transfer detection as a homogeneous DNA diagnostic method
Chen, X., Zehnbauer, B. A., Gnirke, A., et al. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.20.10756 · Cited by 135 · see citing works
dos Remedios, C. G., Miki, M., & Barden, J. A. (1987) · Journal of Muscle Research and Cell Motility · doi:10.1007/bf01753986 · Cited by 119 · see citing works
Day, R. N. (1998) · Molecular Endocrinology · doi:10.1210/mend.12.9.0168 · Cited by 118 · see citing works
Flagged first, then by citations
Research works printed but not linked: 1
- Herman, B. 1998. Fluorescence Microscopy. 2nd ed. Springer-Verlag New York Inc., New York. 170 pp.
Cited by
Sorted by citations
Ghezzi, M., Pescina, S., Padula, C., Santi, P., Del Favero, E., Cantù, L., & Nicoli, S. (2021) · Journal of Controlled Release · doi:10.1016/j.jconrel.2021.02.031 · Cited by 1,135 · see citing works
A global view of protein expression in human cells, tissues, and organs
Pontén, F., Gry, M., Fagerberg, L., Lundberg, E., Asplund, A., Berglund, L., Oksvold, P., Björling, E., Hober, S., Kampf, C., et al. (2009) · Molecular Systems Biology · doi:10.1038/msb.2009.93 · Cited by 222 · see citing works
Advancing pharmaceutical research: A comprehensive review of cutting-edge tools and technologies
Chen, J., Lin, A., & Luo, P. (2024) · Current Pharmaceutical Analysis · doi:10.1016/j.cpan.2024.11.001 · Cited by 112 · see citing works
Peng, H.-Q., Liu, B., Wei, P., Zhang, P., Zhang, H., Zhang, J., Li, K., Li, Y., Cheng, Y., Lam, J. W. Y., et al. (2019) · ACS Nano · doi:10.1021/acsnano.8b08358 · Cited by 94 · see citing works
Moss, F. J., Imoukhuede, P. I., Scott, K., Hu, J., Jankowsky, J. L., Quick, M. W., & Lester, H. A. (2009) · Journal of General Physiology · doi:10.1085/jgp.200910314 · Cited by 45 · see citing works
Pérez de la Mora, M., Borroto-Escuela, D. O., Crespo-Ramírez, M., Rejón-Orantes, J. D. C., Palacios-Lagunas, D. A., Martínez-Mata, M. K., Sánchez-Luna, D., Tesoro-Cruz, E., & Fuxe, K. (2022) · Cells · doi:10.3390/cells11111826 · Cited by 21 · see citing works
Molecular insights into nucleic acid triplexes: methodologies and biological applications
Klose, J. W., Urbina, G., & Pukala, T. L. (2025) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409238.2025.2550728 · Cited by 1 · see citing works
- Authors
- Sekar, R. B., & Periasamy, A.
- Affiliations
- University of Virginia (US)
- Corresponding author
- Periasamy, Ammasi
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-03-03
- Volume
- 160
- Issue
- 5
- Pages
- 629-633
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2173363
- OpenAlex
- W2102557954
- Cited by
- 932
- References
- 29
- Citations by year
- 2026: 32 · 2025: 42 · 2024: 71 · 2023: 58 · 2022: 52 · 2021: 55 · 2020: 52 · 2019: 49 · 2018: 51 · 2017: 33 · 2016: 36 · 2015: 39 · 2014: 34 · 2013: 32 · 2012: 21
- FWCI
- 22.00
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Keywords
- fluorescence resonance energy transfer, live-cell imaging, protein localization, protein-protein interaction, fluorescent probes, super-resolution microscopy
- MeSH terms
- Animals, Eukaryotic Cells, Fluorescent Dyes, Humans, Image Processing, Computer-Assisted, Luminescent Proteins, Microscopy, Fluorescence, Proteins, Fluorescence Resonance Energy Transfer, Green Fluorescent Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review