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Site-Specific Protein Immobilization by Staudinger Ligation
Abstract
The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and immobilized proteins have >80% of their expected activity. No other method enables more rapid immobilization or a higher yield of active protein. Because azido-peptides and azido-proteins are readily attainable by synthesis, biosynthesis, or semisynthesis, the Staudinger ligation could be of unsurpassed utility in creating microarrays of functional peptides and proteins.
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Citations
Cited by
Prescher, J. A., & Bertozzi, C. R. (2005) · Nature Chemical Biology · doi:10.1038/nchembio0605-13 · Cited by 1,456
Enzyme immobilization: an update
Homaei, A. A., Sariri, R., Vianello, F., & Stevanato, R. (2013) · Journal of Chemical Biology · doi:10.1007/s12154-013-0102-9 · Cited by 875
Protein Immobilization Strategies for Protein Biochips
Rusmini, F., Zhong, Z., & Feijen, J. (2007) · Biomacromolecules · doi:10.1021/bm061197b · Cited by 869
Engineering Silicon Oxide Surfaces Using Self‐Assembled Monolayers
Onclin, S., Ravoo, B. J., & Reinhoudt, D. N. (2005) · Angewandte Chemie International Edition · doi:10.1002/anie.200500633 · Cited by 721
The Staudinger Ligation—A Gift to Chemical Biology
Köhn, M., & Breinbauer, R. (2004) · Angewandte Chemie International Edition · doi:10.1002/anie.200401744 · Cited by 585
Metadata
- Authors
- Soellner, M. B., Dickson, K. A., Nilsson, B. L., & Raines, R. T.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-09-04
- Volume
- 125
- Issue
- 39
- Pages
- 11790-11791
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1994696054
- Cited by
- 227
- References
- 0
- FWCI
- 4.50
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Site-Specific Protein Immobilization by Staudinger Ligation. https://app.cochat.ai/papers/10.1021/ja036712h
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- Soellner, M. B., Dickson, K. A., Nilsson, B. L., & Raines, R. T. (2003). Site-Specific Protein Immobilization by Staudinger Ligation. Journal of the American Chemical Society. https://doi.org/10.1021/ja036712h
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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
