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Functionalization of Thioctic Acid-Capped Gold Nanoparticles for Specific Immobilization of Histidine-Tagged Proteins
Open accessAbstract
This paper presents an efficient strategy for the specific immobilization of fully functional proteins onto the surface of nanoparticles. Thioctic acid-derivatized gold clusters are used as a scaffold for further stepwise modification, leading to a cobalt(II)-terminated ligand shell. A histidine tag introduced by genetic engineering into a protein is coordinated to this transition metal ion. The specific immobilization has been demonstrated for the cases of a genetically engineered horseradish peroxidase and ferredoxin-NADP(+) reductase, confirming the attachment of the fully functional proteins to the Co(II)-terminated nanointerface. The absence of nonspecific protein adsorption and the specificity of the binding site have been verified using several analogues of the enzymes without the histidine tag.
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Cited by
Applications of Nanoparticles in Biology
De, M., Ghosh, P. S., & Rotello, V. M. (2008) · Advanced Materials · doi:10.1002/adma.200703183 · Cited by 1,547
Laser ablation synthesis in solution and size manipulation of noble metal nanoparticles
Amendola, V., & Meneghetti, M. (2009) · Physical Chemistry Chemical Physics · doi:10.1039/b900654k · Cited by 1,020
Algar, W. R., Prasuhn, D. E., Stewart, M. H., Jennings, T. L., Blanco-Canosa, J. B., Dawson, P. E., & Medintz, I. L. (2011) · Bioconjugate Chemistry · doi:10.1021/bc200065z · Cited by 502
Therapeutic platforms based on gold nanoparticles and their covalent conjugates with drug molecules
Vigderman, L., & Zubarev, E. R. (2013) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2012.05.004 · Cited by 305
Amendola, V., & Meneghetti, M. (2007) · Journal of Materials Chemistry · doi:10.1039/b709621f · Cited by 150
Metadata
- Authors
- Abad, J. M., Mertens, S. F. L., Pita, M., Fernández, V. M., & Schiffrin, D. J.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-03-19
- Volume
- 127
- Issue
- 15
- Pages
- 5689-5694
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2014811157
- Cited by
- 256
- References
- 0
- FWCI
- 4.84
- Type
- journal article
- Language
- en
- Open access
- green
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- Abad, J. M., Mertens, S. F. L., Pita, M., Fernández, V. M., & Schiffrin, D. J. (2005). Functionalization of Thioctic Acid-Capped Gold Nanoparticles for Specific Immobilization of Histidine-Tagged Proteins. Journal of the American Chemical Society. https://doi.org/10.1021/ja042717i
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