The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Hybrid Quantum Dot−Antibody Fragment Fluorescence Resonance Energy Transfer-Based TNT Sensor
Abstract
We demonstrate the use of luminescent QDs conjugated to antibody fragments to develop solution-phase nanoscale sensing assemblies, based on fluorescence resonance energy transfer (FRET) for the specific detection of the explosive 2,4,6-trinitrotoluene (TNT) in aqueous environments. The hybrid sensor consists of anti-TNT specific antibody fragments attached to a hydrophilic QD via metal-affinity coordination. A dye-labeled TNT analogue prebound in the antibody binding site quenches the QD photoluminescence via proximity-induced FRET. Analysis of the data collected at increasing dye-labeled analogue to QD ratios provided an insight into understanding how the antibody fragments self-assemble on the QD. Addition of soluble TNT displaces the dye-labeled analogue, eliminating FRET and resulting in a concentration-dependent recovery of QD photoluminescence. Sensor performance and specificity were evaluated.
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Cites
Dabbousi, B. O., Rodriguez-Viejo, J., Mikulec, F. V., Heine, J. R., Mattoussi, H., Ober, R., Jensen, K. F., & Bawendi, M. G. (1997) · The Journal of Physical Chemistry B · doi:10.1021/jp971091y · Cited by 4,229
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Cited by
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Sun, X., Wang, Y., & Lei, Y. (2015) · Chemical Society Reviews · doi:10.1039/c5cs00496a · Cited by 1,243
Dasary, S. S. R., Singh, A. K., Senapati, D., Yu, H., & Ray, P. C. (2009) · Journal of the American Chemical Society · doi:10.1021/ja905134d · Cited by 722
CdSe nanocrystal based chem-/bio- sensors
Somers, R. C., Bawendi, M. G., & Nocera, D. G. (2007) · Chemical Society Reviews · doi:10.1039/b517613c · Cited by 616
The use of luminescent quantum dots for optical sensing
Costa-Fernández, J. M., Pereiro, R., & Sanz-Medel, A. (2006) · TrAC Trends in Analytical Chemistry · doi:10.1016/j.trac.2005.07.008 · Cited by 527
Biosensing with Luminescent Semiconductor Quantum Dots
Sapsford, K. E., Pons, T., Medintz, I. L., & Mattoussi, H. (2006) · Sensors · doi:10.3390/s6080925 · Cited by 420
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Metadata
- Authors
- Goldman, E. R., Medintz, I. L., Whitley, J. L., Hayhurst, A., Clapp, A. R., Uyeda, H. T., Deschamps, J. R., Lassman, M. E., & Mattoussi, H.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-04-13
- Volume
- 127
- Issue
- 18
- Pages
- 6744-6751
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2066569554
- Cited by
- 571
- References
- 1
- FWCI
- 29.02
- Type
- journal article
- Language
- en
- Open access
- closed
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