An in Vitro Selection System for TNA
Open accessAbstract
(3‘-2‘)-α-l-Threose nucleic acid (TNA) is an unnatural polymer that possesses the rare ability to base-pair with RNA, DNA, and itself. This feature, coupled with its chemical simplicity, makes TNA of interest as a possible progenitor of RNA during the early history of life. To evaluate the functional potential of TNA, we have developed a system for the in vitro selection of TNA. We identified the Therminator DNA polymerase as a remarkably efficient DNA-dependent TNA polymerase capable of polymerizing more than 50 tNTPs. We have also developed a method of covalently linking a DNA template to the TNA strand that it encodes, thus obviating the need for a TNA-dependent DNA polymerase during cycles of selection.
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Stalling after mismatches can be important for increasing information capacity by essentially reducing production of erroneous full-length copies, if polymerization can occur only during a limited time window.
“Stalling after mismatches can be important for increasing information capacity by essentially reducing production of erroneous full-length copies, if polymerization can occur only during a limited time window.40”
From Cascade of reduced speed and accuracy after errors in enzyme-free copying of nucleic acid sequences (2013) · cites this paper as [40]
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- Authors
- Ichida, J. K., Zou, K., Horhota, A., Yu, B., McLaughlin, L. W., & Szostak, J. W.
- Affiliations
- Boston College (US)
- Corresponding author
- Szostak, Jack W.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-02-10
- Volume
- 127
- Issue
- 9
- Pages
- 2802-2803
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- PMC
- PMC5072288
- OpenAlex
- W1988721235
- Cited by
- 103
- References
- 11
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 8 · 2022: 7 · 2021: 3 · 2019: 2 · 2018: 3 · 2017: 6 · 2016: 2 · 2015: 5 · 2014: 5 · 2013: 6 · 2012: 9
- FWCI
- 3.64
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Journal of the American Chemical Society (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation (0077667)
- National Aeronautics and Space Administration (NNA04CC12A)
- NASA Astrobiology Institute
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM053936)
- Topics
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- TNA, threose nucleic acid, in vitro selection, RNA world, origin of life
- MeSH terms
- Base Sequence, DNA, DNA-Directed DNA Polymerase, DNA, Single-Stranded, Molecular Sequence Data, Nucleic Acid Conformation, Tetroses, Archaeal Proteins
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