New ligase-derived RNA polymerase ribozymes
Open accessAbstract
The search is underway for a catalytic RNA molecule capable of self-replication. Finding such a ribozyme would lend crucial support to the RNA World hypothesis, which holds that very early life-forms relied on RNA for both replicating and storing genetic information. We previously reported an RNA polymerase isolated from a pool of variants of an existing RNA ligase ribozyme. Here we report eight additional ligase-derived polymerase ribozymes isolated from this pool. Because each of them is a new potential starting point for further in vitro evolution and engineering, together they substantially enrich the set of candidates from which an RNA replicase ribozyme might eventually emerge.
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RNA ligation can be accomplished by shorter ribozyme sequences compared to the polymerase, and the first RNA polymerase was evolved from the Class I ligase.
“Interestingly, RNA ligation can be accomplished by shorter ribozyme sequences compared to the polymerase (and the first RNA polymerase was evolved from the Class I ligase [95,101]).”
From The RNA world as a model system to study the origin of life (2015) · cites this paper as [101]
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- Authors
- LAWRENCE, M. S., & BARTEL, D. P.
- Affiliations
- Whitehead Institute for Biomedical Research (US)
- Venue
- RNA
- Publisher
- Cold Spring Harbor Laboratory
- Published
- 2005-06-29
- Volume
- 11
- Issue
- 8
- Pages
- 1173-1180
- ISSN
- 1355-8382 (ISSN-L)
- 1469-9001
- PMC
- PMC1370801
- OpenAlex
- W2105801230
- Cited by
- 68
- References
- 49
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 4 · 2023: 2 · 2022: 1 · 2021: 1 · 2019: 3 · 2017: 1 · 2015: 4 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 4.07
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- RNA (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Funders
- National Institutes of Health
- Topics
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Evolution and Genetic Dynamics
- Keywords
- RNA replicase, RNA world, in vitro evolution, ribozymes, self-replication
- MeSH terms
- Base Sequence, Cloning, Molecular, Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, RNA Ligase (ATP), Recombinant Proteins, RNA, DNA-Directed RNA Polymerases, RNA, Catalytic
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