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Kinase-Catalyzed Modification of Gold Nanoparticles: A New Approach to Colorimetric Kinase Activity Screening
Abstract
Peptide-stabilized gold nanoparticles have been enzymatically biotinylated by a kinase-catalyzed reaction using biotin-ATP as a cosubstrate. Upon mixing with avidin-modified particles, solutions of biotinylated particles change color from red to blue, indicating aggregation of particles. On the basis of this reaction, we have developed a simple colorimetric test to monitor kinase inhibitor activity.
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Citations
Cited by
Applications of Nanoparticles in Biology
De, M., Ghosh, P. S., & Rotello, V. M. (2008) · Advanced Materials · doi:10.1002/adma.200703183 · Cited by 1,547
Song, J., Zhou, J., & Duan, H. (2012) · Journal of the American Chemical Society · doi:10.1021/ja305154a · Cited by 438
A Review of Gold and Silver Nanoparticle‐Based Colorimetric Sensing Assays
Sabela, M., Balme, S., Bechelany, M., Janot, J., & Bisetty, K. (2017) · Advanced Engineering Materials · doi:10.1002/adem.201700270 · Cited by 312
Gold nanoparticles: an era in bionanotechnology
Kumar, D., Saini, N., Jain, N., Sareen, R., & Pandit, V. (2013) · Expert Opinion on Drug Delivery · doi:10.1517/17425247.2013.749854 · Cited by 96
Metadata
- Authors
- Wang, Z., Lévy, R., Fernig, D. G., & Brust, M.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-01-31
- Volume
- 128
- Issue
- 7
- Pages
- 2214-2215
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2151190288
- Cited by
- 276
- References
- 0
- FWCI
- 12.92
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Kinase-Catalyzed Modification of Gold Nanoparticles: A New Approach to Colorimetric Kinase Activity Screening. https://app.cochat.ai/papers/10.1021/ja058135y
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- Wang, Z., Lévy, R., Fernig, D. G., & Brust, M. (2006). Kinase-Catalyzed Modification of Gold Nanoparticles: A New Approach to Colorimetric Kinase Activity Screening. Journal of the American Chemical Society. https://doi.org/10.1021/ja058135y
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
