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Cited by 270

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Quantitative and Comprehensive Decomposition of the Ion Atmosphere around Nucleic Acids

Open access
Bai, Yu, Greenfeld, Max, Travers, Kevin J., Chu, Vincent B., Lipfert, Jan, Doniach, Sebastian, Herschlag, Daniel·2007·Journal of the American Chemical Society
DOI 10.1021/ja075020g
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Abstract

The ion atmosphere around nucleic acids critically affects biological and physical processes such as chromosome packing, RNA folding, and molecular recognition. However, the dynamic nature of the ion atmosphere renders it difficult to characterize. The basic thermodynamic description of this atmosphere, a full accounting of the type and number of associated ions, has remained elusive. Here we provide the first complete accounting of the ion atmosphere, using buffer equilibration and atomic emission spectroscopy (BE-AES) to accurately quantitate the cation association and anion depletion. We have examined the influence of ion size and charge on ion occupancy around simple, well-defined DNA molecules. The relative affinity of monovalent and divalent cations correlates inversely with their size. Divalent cations associate preferentially over monovalent cations; e.g., with Na+ in 4-fold excess of Mg2+ (20 vs 5 mM), the ion atmosphere nevertheless has 3-fold more Mg2+ than Na+. Further, the dicationic polyamine putrescine2+ does not compete effectively for association relative to divalent metal ions, presumably because of its lower charge density. These and other BE-AES results can be used to evaluate and guide the improvement of electrostatic treatments. As a first step, we compare the BE-AES results to predictions from the widely used nonlinear Poisson Boltzmann (NLPB) theory and assess the applicability and precision of this theory. In the future, BE-AES in conjunction with improved theoretical models, can be applied to complex binding and folding equilibria of nucleic acids and their complexes, to parse the electrostatic contribution from the overall thermodynamics of important biological processes.

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Metadata

Authors
Bai, Y., Greenfeld, M., Travers, K. J., Chu, V. B., Lipfert, J., Doniach, S., & Herschlag, D.
Venue
Journal of the American Chemical Society
Publisher
American Chemical Society (ACS)
Published
2007-11-09
Volume
129
Issue
48
Pages
14981-14988
ISSN
  • 0002-7863 (ISSN-L)
  • 1520-5126
DOI
10.1021/ja075020g
OpenAlex
W1982913950
Cited by
270
References
30
FWCI
7.80
Type
journal article
Language
en
Open access
green

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