The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Superresolution Imaging of Targeted Proteins in Fixed and Living Cells Using Photoactivatable Organic Fluorophores
Open accessAbstract
Superresolution imaging techniques based on sequential imaging of sparse subsets of single molecules require fluorophores whose emission can be photoactivated or photoswitched. Because typical organic fluorophores can emit significantly more photons than average fluorescent proteins, organic fluorophores have a potential advantage in super-resolution imaging schemes, but targeting to specific cellular proteins must be provided. We report the design and application of HaloTag-based target-specific azido DCDHFs, a class of photoactivatable push-pull fluorogens which produce bright fluorescent labels suitable for single-molecule superresolution imaging in live bacterial and fixed mammalian cells.
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Citations
Cites
Precise Nanometer Localization Analysis for Individual Fluorescent Probes
Thompson, R. E., Larson, D. R., & Webb, W. W. (2002) · Biophysical Journal · doi:10.1016/s0006-3495(02)75618-x · Cited by 2,320
Cited by
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging
Dempsey, G. T., Vaughan, J. C., Chen, K. H., Bates, M., & Zhuang, X. (2011) · Nature Methods · doi:10.1038/nmeth.1768 · Cited by 1,369
Breaking the Diffraction Barrier: Super-Resolution Imaging of Cells
Huang, B., Babcock, H., & Zhuang, X. (2010) · Cell · doi:10.1016/j.cell.2010.12.002 · Cited by 1,156
Direct stochastic optical reconstruction microscopy with standard fluorescent probes
van de Linde, S., Löschberger, A., Klein, T., Heidbreder, M., Wolter, S., Heilemann, M., & Sauer, M. (2011) · Nature Protocols · doi:10.1038/nprot.2011.336 · Cited by 1,071
Bright photoactivatable fluorophores for single-molecule imaging
Grimm, J. B., English, B. P., Choi, H., Muthusamy, A. K., Mehl, B. P., Dong, P., Brown, T. A., Lippincott-Schwartz, J., Liu, Z., Lionnet, T., et al. (2016) · Nature Methods · doi:10.1038/nmeth.4034 · Cited by 435
Switchable Fluorophores for Single-Molecule Localization Microscopy
Li, H., & Vaughan, J. C. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00767 · Cited by 319
Metadata
- Authors
- Lee, H.-L. D., Lord, S. J., Iwanaga, S., Zhan, K., Xie, H., Williams, J. C., Wang, H., Bowman, G. R., Goley, E. D., Shapiro, L., Twieg, R. J., Rao, J., & Moerner, W. E.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-10-11
- Volume
- 132
- Issue
- 43
- Pages
- 15099-15101
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2010468931
- Cited by
- 175
- References
- 1
- FWCI
- 10.19
- Type
- journal article
- Language
- en
- Open access
- green
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