The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Protein Crowding Tunes Protein Stability
Abstract
Thirty percent of a cell's volume is filled with macromolecules, and protein chemistry in a crowded environment is predicted to differ from that in dilute solution. We quantified the effect of crowding by globular proteins on the equilibrium thermodynamic stability of a small globular protein. Theory has long predicted that crowding should stabilize proteins, and experiments using synthetic polymers as crowders show such stabilizing effects. We find that protein crowders can be mildly destabilizing. The destabilization arises from a competition between stabilizing excluded-volume effects and destabilizing nonspecific interactions, including electrostatic interactions. This competition results in tunable stability, which could impact our understanding of the spatial and temporal roles of proteins in living systems.
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Citations
Cites
Excluded volume as a determinant of macromolecular structure and reactivity
Minton, A. P. (1981) · Biopolymers · doi:10.1002/bip.1981.360201006 · Cited by 589
Cited by
Macromolecular Crowding In Vitro , In Vivo , and In Between
Rivas, G., & Minton, A. P. (2016) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2016.08.013 · Cited by 470
Wei, G., Xi, W., Nussinov, R., & Ma, B. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00562 · Cited by 436
Macromolecular Crowding and Protein Stability
Wang, Y., Sarkar, M., Smith, A. E., Krois, A. S., & Pielak, G. J. (2012) · Journal of the American Chemical Society · doi:10.1021/ja305300m · Cited by 346
Self-citationRational design of 19F NMR labelling sites to probe protein structure and interactions
Streit, J. O., Chan, S. H. S., Daya, S., & Christodoulou, J. (2025) · Nature Communications · doi:10.1038/s41467-025-59105-6 · Cited by 17
Metadata
- Authors
- Miklos, A. C., Sarkar, M., Wang, Y., & Pielak, G. J.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-04-20
- Volume
- 133
- Issue
- 18
- Pages
- 7116-7120
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2325407743
- Cited by
- 296
- References
- 1
- FWCI
- 9.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- Miklos, A. C., Sarkar, M., Wang, Y., & Pielak, G. J. (2011). Protein Crowding Tunes Protein Stability. Journal of the American Chemical Society. https://doi.org/10.1021/ja200067p
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