The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Imaging Intracellular pH in Live Cells with a Genetically Encoded Red Fluorescent Protein Sensor
Open accessAbstract
Intracellular pH affects protein structure and function, and proton gradients underlie the function of organelles such as lysosomes and mitochondria. We engineered a genetically encoded pH sensor by mutagenesis of the red fluorescent protein mKeima, providing a new tool to image intracellular pH in live cells. This sensor, named pHRed, is the first ratiometric, single-protein red fluorescent sensor of pH. Fluorescence emission of pHRed peaks at 610 nm while exhibiting dual excitation peaks at 440 and 585 nm that can be used for ratiometric imaging. The intensity ratio responds with an apparent pK(a) of 6.6 and a >10-fold dynamic range. Furthermore, pHRed has a pH-responsive fluorescence lifetime that changes by ~0.4 ns over physiological pH values and can be monitored with single-wavelength two-photon excitation. After characterizing the sensor, we tested pHRed's ability to monitor intracellular pH by imaging energy-dependent changes in cytosolic and mitochondrial pH.
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Cites
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Sensors and regulators of intracellular pH
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Fluorescent Indicators for Intracellular pH
Han, J., & Burgess, K. (2010) · Chemical Reviews · doi:10.1021/cr900249z · Cited by 1,620
Genetically encoded fluorescent indicator for intracellular hydrogen peroxide
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Cited by
Ryu, D., Mouchiroud, L., Andreux, P. A., Katsyuba, E., Moullan, N., Nicolet-dit-Félix, A. A., Williams, E. G., Jha, P., Lo Sasso, G., Huzard, D., et al. (2016) · Nature Medicine · doi:10.1038/nm.4132 · Cited by 1,105
Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications
Steinegger, A., Wolfbeis, O. S., & Borisov, S. M. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.0c00451 · Cited by 622
Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor
Hung, Y. P., Albeck, J. G., Tantama, M., & Yellen, G. (2011) · Cell Metabolism · doi:10.1016/j.cmet.2011.08.012 · Cited by 509
Neuronal Stimulation Triggers Neuronal Glycolysis and Not Lactate Uptake
Díaz-García, C. M., Mongeon, R., Lahmann, C., Koveal, D., Zucker, H., & Yellen, G. (2017) · Cell Metabolism · doi:10.1016/j.cmet.2017.06.021 · Cited by 466
Self-citationChen, S., Hong, Y., Liu, Y., Liu, J., Leung, C. W. T., Li, M., Kwok, R. T. K., Zhao, E., Lam, J. W. Y., Yu, Y., et al. (2013) · Journal of the American Chemical Society · doi:10.1021/ja400337p · Cited by 434
Metadata
- Authors
- Tantama, M., Hung, Y. P., & Yellen, G.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-06-09
- Volume
- 133
- Issue
- 26
- Pages
- 10034-10037
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2027631918
- Cited by
- 408
- References
- 30
- FWCI
- 13.88
- Type
- journal article
- Language
- en
- Open access
- green
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