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Specific Protein Detection Using Designed DNA Carriers and Nanopores
Open accessAbstract
Nanopores are a versatile technique for the detection and characterization of single molecules in solution. An ongoing challenge in the field is to find methods to selectively detect specific biomolecules. In this work we describe a new technique for sensing specific proteins using unmodified solid-state nanopores. We engineered a double strand of DNA by hybridizing nearly two hundred oligonucleotides to a linearized version of the m13mp18 virus genome. This engineered double strand, which we call a DNA carrier, allows positioning of protein binding sites at nanometer accurate intervals along its contour via DNA conjugation chemistry. We measure the ionic current signal of translocating DNA carriers as a function of the number of binding sites and show detection down to the single protein level. Furthermore, we use DNA carriers to develop an assay for identifying a single protein species within a protein mixture.
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Cited by
Nanopore-based technologies beyond DNA sequencing
Ying, Y.-L., Hu, Z.-L., Zhang, S., Qing, Y., Fragasso, A., Maglia, G., Meller, A., Bayley, H., Dekker, C., & Long, Y.-T. (2022) · Nature Nanotechnology · doi:10.1038/s41565-022-01193-2 · Cited by 538
Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores
Bell, N. A. W., & Keyser, U. F. (2016) · Nature Nanotechnology · doi:10.1038/nnano.2016.50 · Cited by 345
Self-citationSingle-molecule protein sensing in a nanopore: a tutorial
Varongchayakul, N., Song, J., Meller, A., & Grinstaff, M. W. (2018) · Chemical Society Reviews · doi:10.1039/c8cs00106e · Cited by 282
Digital Data Storage Using DNA Nanostructures and Solid-State Nanopores
Chen, K., Kong, J., Zhu, J., Ermann, N., Predki, P., & Keyser, U. F. (2019) · Nano Letters · doi:10.1021/acs.nanolett.8b04715 · Cited by 164
Self-citationNanopore-Based DNA Hard Drives for Rewritable and Secure Data Storage
Chen, K., Zhu, J., Bošković, F., & Keyser, U. F. (2020) · Nano Letters · doi:10.1021/acs.nanolett.0c00755 · Cited by 134
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Metadata
- Authors
- Bell, N. A. W., & Keyser, U. F.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2015-02-03
- Volume
- 137
- Issue
- 5
- Pages
- 2035-2041
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W2058329064
- Cited by
- 202
- References
- 0
- FWCI
- 11.17
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Bell, N. A. W., & Keyser, U. F. (2015). Specific Protein Detection Using Designed DNA Carriers and Nanopores. Journal of the American Chemical Society. https://doi.org/10.1021/ja512521w
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