Digital Data Storage Using DNA Nanostructures and Solid-State Nanopores
Open accessAbstract
Solid-state nanopores are powerful tools for reading the three-dimensional shape of molecules, allowing for the translation of molecular structure information into electric signals. Here, we show a high-resolution integrated nanopore system for identifying DNA nanostructures that has the capability of distinguishing attached short DNA hairpins with only a stem length difference of 8 bp along a DNA double strand named the DNA carrier. Using our platform, we can read up to 112 DNA hairpins with a separating distance of 114 bp attached on a DNA carrier that carries digital information. Our encoding strategy allows for the creation of a library of molecules with a size of up to 5 × 10 33 (2 112 ) that is only built from a few hundred types of base molecules for data storage and has the potential to be extended by linking multiple DNA carriers. Our platform provides a nanopore- and DNA nanostructure-based data storage method with convenient access and the potential for miniature-scale integration.
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Information can be stored in the 3D structures of DNA assemblies instead of in the sequence, with readout relying on imaging techniques or single-molecule nanopore measurements.
“Information can thus be stored in the 3D structures of these assemblies instead of in the sequence, with readout relying on imaging techniques, such as super resolution imaging,22 or using single-molecule nanopore measurements.23,25”
From Emerging Approaches to DNA Data Storage: Challenges and Prospects (2022) · cites this paper as [23]
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- Authors
- Chen, K., Kong, J., Zhu, J., Ermann, N., Predki, P., & Keyser, U. F.
- Affiliations
- University of Cambridge (GB)
- Iridia Incorporated, 3156 Lionshead Avenue, Suite 1, Carlsbad, California 92010, United States
- Corresponding author
- Keyser, Ulrich F.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2018-12-26
- Volume
- 19
- Issue
- 2
- Pages
- 1210-1215
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W2906493741
- Cited by
- 164
- References
- 41
- Citations by year
- 2026: 12 · 2025: 24 · 2024: 18 · 2023: 34 · 2022: 22 · 2021: 23 · 2020: 19 · 2019: 12
- FWCI
- 5.39
- Type
- journal article
- Language
- en
- Open access
- Locations
- Nano Letters (publishedVersion)
- PubMed (publishedVersion)
- Apollo (University of Cambridge) (submittedVersion)
- Apollo
- Funders
- Engineering and Physical Sciences Research Council (EP/M008258/1)
- Cambridge Commonwealth, European and International Trust
- H2020 European Research Council (647144)
- Trinity Hall, University of Cambridge
- Iridia, Inc.
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Nanopore and Nanochannel Transport Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Biological Computing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Keywords
- DNA nanostructures, solid-state nanopores, DNA data storage, DNA hairpins, DNA carriers, nanopore sequencing, molecular encoding, DNA nanotechnology
- MeSH terms
- Base Sequence, DNA, Electricity, Gene Library, Information Storage and Retrieval, Nanotechnology, Nanostructures, Nanopores
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