Cited by 279

The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.

Formation of a Unique End-to-End Stacked Pair of G-Quadruplexes in the hTERT Core Promoter with Implications for Inhibition of Telomerase by G-Quadruplex-Interactive Ligands

Open access

Palumbo, SunMi L., Ebbinghaus, Scot W., Hurley, Laurence H.·2009·Journal of the American Chemical Society

Open this paper in CoChat — free account

Abstract

The hTERT core promoter contains a G-rich region of 12 consecutive G-tracts, embracing 3 Sp1 binding sites, and has the potential to form multiple G-quadruplexes. From the 12 runs of guanines, 9 putative hTERT G-quadruplex-forming sequences were selected to assay for G-quadruplex formation and stability using circular dichroism and a Taq polymerase stop assay. Results from biophysical and chemical assays demonstrate an approximate inverse correlation between total loop size and structure stability. Investigation of the full-length hTERT G-rich sequence using a Taq polymerase stop assay and dimethyl sulfate footprinting revealed the formation of a unique end-to-end stacked G-quadruplex structure from this sequence. This structure consists of an all parallel G-quadruplex, formed by four consecutive G-tracts, linked to another, atypical G-quadruplex, formed by two pairs of consecutive G-tracts separated by a 26-base loop. This 26-base loop likely forms a stable hairpin structure, which would explain the unexpected stability of this G-quadruplex. Significantly, the formation of this tandem G-quadruplex structure in the full-length sequence masks all three Sp1 binding sites, which is predicted to produce significant inhibition of hTERT promoter activity. Furthermore, our study implies that inhibition of telomerase activity by some G-quadruplex ligands is not only produced by targeting telomeric G-quadruplexes but also by stabilization of the hTERT promoter G-quadruplexes.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1021/ja902281d

Cites

60 of 60 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Direct evidence for a G-quadruplex in a promoter region and its targeting with a small molecule to repress c- MYC transcription
Siddiqui-Jain, A., Grand, C. L., Bearss, D. J., & Hurley, L. H. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.182256799 · Cited by 2,238
Self-citation
The Myc/Max/Mad Network and the Transcriptional Control of Cell Behavior
Grandori, C., Cowley, S. M., James, L. P., & Eisenman, R. N. (2000) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.16.1.653 · Cited by 1,333
Human telomeric sequence forms a hybrid-type intramolecular G-quadruplex structure with mixed parallel/antiparallel strands in potassium solution
Ambrus, A., Chen, D., Dai, J., Bialis, T., Jones, R. A., & Yang, D. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl348 · Cited by 1,106
Inhibition of Human Telomerase by a G-Quadruplex-Interactive Compound
Sun, D., Thompson, B., Cathers, B. E., Salazar, M., Kerwin, S. M., Trent, J. O., Jenkins, T. C., Neidle, S., & Hurley, L. H. (1997) · Journal of Medicinal Chemistry · doi:10.1021/jm970199z · Cited by 794
Self-citation
G-quadruplex formation within the promoter of the KRAS proto-oncogene and its effect on transcription
Cogoi, S., & Xodo, L. E. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl286 · Cited by 739
Solution Structure of the Biologically Relevant G-Quadruplex Element in the Human c-MYC Promoter. Implications for G-Quadruplex Stabilization
Ambrus, A., Chen, D., Dai, J., Jones, R. A., & Yang, D. (2005) · Biochemistry · doi:10.1021/bi048242p · Cited by 683
The G-Quadruplex-Interactive Molecule BRACO-19 Inhibits Tumor Growth, Consistent with Telomere Targeting and Interference with Telomerase Function
Burger, A. M., Dai, F., Schultes, C. M., Reszka, A. P., Moore, M. J., Double, J. A., & Neidle, S. (2005) · Cancer Research · doi:10.1158/0008-5472.can-04-2910 · Cited by 587
Telomestatin, a Novel Telomerase Inhibitor from Streptomyces anulatus
Shin-ya, K., Wierzba, K., Matsuo, K.-I., Ohtani, T., Yamada, Y., Furihata, K., Hayakawa, Y., & Seto, H. (2001) · Journal of the American Chemical Society · doi:10.1021/ja005780q · Cited by 509
Propeller-Type Parallel-Stranded G-Quadruplexes in the Human c-myc Promoter
Phan, A. T., Modi, Y. S., & Patel, D. J. (2004) · Journal of the American Chemical Society · doi:10.1021/ja048805k · Cited by 501
Loop-Length-Dependent Folding of G-Quadruplexes
Hazel, P., Huppert, J., Balasubramanian, S., & Neidle, S. (2004) · Journal of the American Chemical Society · doi:10.1021/ja045154j · Cited by 494
Sp1 cooperates with c-Myc to activate transcription of the human telomerase reverse transcriptase gene (hTERT)
Kyo, S. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.3.669 · Cited by 494
The human telomerase catalytic subunit hTERT: organization of the gene and characterization of the promoter
Cong, Y. (1999) · Human Molecular Genetics · doi:10.1093/hmg/8.1.137 · Cited by 485
Cloning of human telomerase catalytic subunit(hTERT)gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells
高倉, 正. (2002) · Medical Entomology and Zoology · Cited by 475
Structures, folding patterns, and functions of intramolecular DNA G-quadruplexes found in eukaryotic promoter regions
Qin, Y., & Hurley, L. H. (2008) · Biochimie · doi:10.1016/j.biochi.2008.02.020 · Cited by 450
Self-citation
The Importance of Negative Superhelicity in Inducing the Formation of G-Quadruplex and i-Motif Structures in the c-Myc Promoter: Implications for Drug Targeting and Control of Gene Expression
Sun, D., & Hurley, L. H. (2009) · Journal of Medicinal Chemistry · doi:10.1021/jm900055s · Cited by 397
Self-citation
Cloning and characterization of the promoter region of human telomerase reverse transcriptase gene.
Horikawa, I., Cable, P. L., Afshari, C. A., & Barrett, J. C. (1999) · PubMed · Cited by 393
Human POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro
Zaug, A. J., Podell, E. R., & Cech, T. R. (2005) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0504744102 · Cited by 385
Deconvoluting the Structural and Drug-Recognition Complexity of the G-Quadruplex-Forming Region Upstream of the bcl-2 P1 Promoter
Dexheimer, T. S., Sun, D., & Hurley, L. H. (2006) · Journal of the American Chemical Society · doi:10.1021/ja0563861 · Cited by 383
Self-citation
NMR solution structure of the major G-quadruplex structure formed in the human BCL2 promoter region
Dai, J., Chen, D., Jones, R. A., Hurley, L. H., & Yang, D. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl610 · Cited by 373
Self-citation
The cationic porphyrin TMPyP4 down-regulates c-MYC and human telomerase reverse transcriptase expression and inhibits tumor growth in vivo.
Grand, C. L., Han, H., Muñoz, R. M., Weitman, S., Hoff, D. D. V., Hurley, L. H., & Bearss, D. J. (2002) · PubMed · Cited by 368
Self-citation
Stabilization of G-Quadruplex DNA and Inhibition of Telomerase Activity by Square-Planar Nickel(II) Complexes
Reed, J. E., Arnal, A. A., Neidle, S., & Vilar, R. (2006) · Journal of the American Chemical Society · doi:10.1021/ja058509n · Cited by 338
A Sequence-Independent Study of the Influence of Short Loop Lengths on the Stability and Topology of Intramolecular DNA G-Quadruplexes
Bugaut, A., & Balasubramanian, S. (2008) · Biochemistry · doi:10.1021/bi701873c · Cited by 322
Defective repression of c- myc in breast cancer cells: A loss at the core of the transforming growth factor β growth arrest program
Chen, C.-R., Kang, Y., & Massagué, J. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.98.3.992 · Cited by 320
A G-Quadruplex-Interactive Potent Small-Molecule Inhibitor of Telomerase Exhibiting in Vitro and in Vivo Antitumor Activity
Gowan, S. M., Harrison, J. R., Patterson, L., Valenti, M., Read, M. A., Neidle, S., & Kelland, L. R. (2002) · Molecular Pharmacology · doi:10.1016/s0026-895x(24)12212-2 · Cited by 303
Evidence for the Presence of a Guanine Quadruplex Forming Region within a Polypurine Tract of the Hypoxia Inducible Factor 1α Promoter
De Armond, R., Wood, S., Sun, D., Hurley, L. H., & Ebbinghaus, S. W. (2005) · Biochemistry · doi:10.1021/bi051618u · Cited by 285
Self-citation
Downregulation of telomerase reverse transcriptase mRNA expression by wild type p53 in human tumor cells
Xu, D., Wang, Q., Gruber, A., Björkholm, M., Chen, Z., Zaid, A., Selivanova, G., Peterson, C., Wiman, K. G., & Pisa, P. (2000) · Oncogene · doi:10.1038/sj.onc.1203890 · Cited by 257
Overcoming the immortality of tumour cells by telomere and telomerase based cancer therapeutics – current status and future prospects
Kelland, L. R. (2005) · European Journal of Cancer · doi:10.1016/j.ejca.2004.11.024 · Cited by 252
c-myc Is a Downstream Target of the Smad Pathway
Yagi, K., Furuhashi, M., Aoki, H., Goto, D., Kuwano, H., Sugamura, K., Miyazono, K., & Kato, M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m104170200 · Cited by 244
G-Quadruplex stabilization by telomestatin induces TRF2 protein dissociation from telomeres and anaphase bridge formation accompanied by loss of the 3′ telomeric overhang in cancer cells
Tahara, H., Shin-ya, K., Seimiya, H., Yamada, H., Tsuruo, T., & Ide, T. (2006) · Oncogene · doi:10.1038/sj.onc.1209217 · Cited by 239
Influence of loop size on the stability of intramolecular DNA quadruplexes
Risitano, A. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh598 · Cited by 237
Telomerase downregulation induced by the G-quadruplex ligand 12459 in A549 cells is mediated by hTERT RNA alternative splicing
Gomez, D. (2004) · Nucleic Acids Research · doi:10.1093/nar/gkh181 · Cited by 236
Ethidium derivatives bind to G-quartets, inhibit telomerase and act as fluorescent probes for quadruplexes
Koeppel, F. (2001) · Nucleic Acids Research · doi:10.1093/nar/29.5.1087 · Cited by 221
Telomerase inhibition with a novel G-quadruplex-interactive agent, telomestatin: in vitro and in vivo studies in acute leukemia
Tauchi, T., Shin-ya, K., Sashida, G., Sumi, M., Okabe, S., Ohyashiki, J. H., & Ohyashiki, K. (2006) · Oncogene · doi:10.1038/sj.onc.1209577 · Cited by 218
Activity of a novel G-quadruplex-interactive telomerase inhibitor, telomestatin (SOT-095), against human leukemia cells: involvement of ATM-dependent DNA damage response pathways
Tauchi, T., Shin-ya, K., Sashida, G., Sumi, M., Nakajima, A., Shimamoto, T., Ohyashiki, J. H., & Ohyashiki, K. (2003) · Oncogene · doi:10.1038/sj.onc.1206833 · Cited by 206
The G-quadruplex Ligand Telomestatin Inhibits POT1 Binding to Telomeric Sequences In vitro and Induces GFP-POT1 Dissociation from Telomeres in Human Cells
Gomez, D., O'Donohue, M.-F., Wenner, T., Douarre, C., Macadré, J., Koebel, P., Giraud-Panis, M.-J., Kaplan, H., Kolkes, A., Shin-ya, K., et al. (2006) · Cancer Research · doi:10.1158/0008-5472.can-06-1581 · Cited by 200
Intramolecularly folded G-quadruplex and i-motif structures in the proximal promoter of the vascular endothelial growth factor gene
Guo, K., Gokhale, V., Hurley, L. H., & Sun, D. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn380 · Cited by 190
Self-citation
A novel G-quadruplex-forming GGA repeat region in the c-myb promoter is a critical regulator of promoter activity
Palumbo, S. L., Memmott, R. M., Uribe, D. J., Krotova-Khan, Y., Hurley, L. H., & Ebbinghaus, S. W. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkm1069 · Cited by 183
Self-citation
Telomeres, telomerase and cancer: an up-date
Dhaene, K., Van Marck, E., & Parwaresch, R. (2000) · Virchows Archiv · doi:10.1007/s004280000189 · Cited by 165
Loss of hPot1 Function Leads to Telomere Instability and a cut-like Phenotype
Veldman, T., Etheridge, K. T., & Counter, C. M. (2004) · Current Biology · doi:10.1016/j.cub.2004.12.031 · Cited by 151
Mechanism of acridine-based telomerase inhibition and telomere shortening
Gunaratnam, M., Greciano, O., Martins, C., Reszka, A. P., Schultes, C. M., Morjani, H., Riou, J., & Neidle, S. (2007) · Biochemical Pharmacology · doi:10.1016/j.bcp.2007.06.011 · Cited by 145
Antisense-mediated hTERT inhibition specifically reduces the growth of human bladder cancer cells.
Kraemer, K., Fuessel, S., Schmidt, U., Kotzsch, M., Schwenzer, B., Wirth, M. P., & Meye, A. (2003) · PubMed · Cited by 136
The Tail of the Telomere
Petraccone, L., Trent, J. O., & Chaires, J. B. (2008) · Journal of the American Chemical Society · doi:10.1021/ja8075567 · Cited by 128
Telomerase Inhibition and Cell Growth Arrest After Telomestatin Treatment in Multiple Myeloma
Shammas, M. A., Reis, R. J. S., Cheng, L., Koley, H., Hurley, L. H., Anderson, K. C., & Munshi, N. C. (2004) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-0793-03 · Cited by 119
Self-citation
Antitumor activity of G-quadruplex–interactive agent TMPyP4 in K562 leukemic cells
Mikami-Terao, Y., Akiyama, M., Yuza, Y., Yanagisawa, T., Yamada, O., & Yamada, H. (2007) · Cancer Letters · doi:10.1016/j.canlet.2007.11.017 · Cited by 108
Efficient Inhibition of Human Telomerase Reverse Transcriptase Expression by RNA Interference Sensitizes Cancer Cells to Ionizing Radiation and Chemotherapy
Nakamura, M., Masutomi, K., Kyo, S., Hashimoto, M., Maida, Y., Kanaya, T., Tanaka, M., Hahn, W. C., & Inoue, M. (2005) · Human Gene Therapy · doi:10.1089/hum.2005.16.859 · Cited by 91
Antisense oligonucleotide-mediated inhibition of hTERT, but not hTERC, induces rapid cell growth decline and apoptosis in the absence of telomere shortening in human prostate cancer cells
Folini, M., Brambilla, C., Villa, R., Gandellini, P., Vignati, S., Paduano, F., Daidone, M. G., & Zaffaroni, N. (2005) · European Journal of Cancer · doi:10.1016/j.ejca.2004.12.002 · Cited by 90
Telomerase as a universal tumor antigen for cancer vaccines
Beatty, G. L., & Vonderheide, R. H. (2008) · Expert Review of Vaccines · doi:10.1586/14760584.7.7.881 · Cited by 70
Telomerase Expression in Noncancerous Bronchial Epithelia Is a Possible Marker of Early Development of Lung Cancer
Miyazu, Y., Miyazawa, T., Hiyama, K., Kurimoto, N., Iwamoto, Y., Matsuura, H., Kanoh, K., Kohno, N., Nishiyama, M., & Hiyama, E. (2005) · Cancer Research · doi:10.1158/0008-5472.can-05-0976 · Cited by 62
Consequences of Telomerase Inhibition by BIBR1532 on Proliferation and Chemosensitivity of Chondrosarcoma Cell Lines
Parsch, D., Brassat, U., Brümmendorf, T. H., & Fellenberg, J. (2008) · Cancer Investigation · doi:10.1080/07357900802072905 · Cited by 53
The Immunogenicity of the hTERT540-548 Peptide in Cancer
Wenandy, L., Sørensen, R. B., Sengeløv, L., Svane, I. M., Straten, P. T., & Andersen, M. H. (2008) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-07-4590 · Cited by 46
Telomerase and telomere dynamics in ageing and cancer: current status and future directions
Camarena, F. S., Serral, G. C., & Sampedro, F. (2007) · Clinical & Translational Oncology · doi:10.1007/s12094-007-0028-1 · Cited by 34
Telomerase and cancer.
Harley, C. B., & Kim, N. W. (1996) · PubMed · Cited by 33
A molecular model for drug binding to tandem repeats of telomeric G-quadruplexes
Haider, S. M., & Neidle, S. (2009) · Biochemical Society Transactions · doi:10.1042/bst0370583 · Cited by 30
Efficient inhibition of human telomerase activity by antisense oligonucleotides sensitizes cancer cells to radiotherapy
Ji, X., Xie, C., Fang, M., Zhou, F., Zhang, W., Zhang, M., & Zhou, Y. (2006) · Acta Pharmacologica Sinica · doi:10.1111/j.1745-7254.2006.00417.x · Cited by 30
Inhibition of human telomerase reverse transcriptase gene expression by BRCA1 in human ovarian cancer cells
Zhou, C., & Liu, J. (2003) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(03)00318-8 · Cited by 29
Combination of telomerase antisense oligonucleotides simultaneously targeting hTR and hTERT produces synergism of inhibition of telomerase activity and growth in human colon cancer cell line
Fu, X. (2005) · World Journal of Gastroenterology · doi:10.3748/wjg.v11.i6.785 · Cited by 18
Telomerase as a tumor-associated antigen for cancer immunotherapy
Patel, K. P., & Vonderheide, R. H. (2004) · Cytotechnology · doi:10.1007/s10616-004-5132-2 · Cited by 12
Uses of Telomerase Peptides in Anti-Tumor Immune Therapy
Li, H., Katik, I., & Liu, J. (2007) · Methods in molecular biology · doi:10.1007/978-1-60327-070-0_7 · Cited by 8
Telomerase activity in human germline and embryonic tissues and cells
Wright, W. E., Piatyszek, M. A., Rainey, W. E., Byrd, W., & Shay, J. W. (1996) · Developmental Genetics · doi:10.1002/(sici)1520-6408(1996)18:2<173::aid-dvg10>3.0.co;2-3
Untitled work
(1999) · PubMed

DNA secondary structures: stability and function of G-quadruplex structures
Bochman, M. L., Paeschke, K., & Zakian, V. A. (2012) · Nature Reviews Genetics · doi:10.1038/nrg3296 · Cited by 1,446
The transcription factor GABP selectively binds and activates the mutant TERT promoter in cancer
Bell, R. J. A., Rube, H. T., Kreig, A., Mancini, A., Fouse, S. D., Nagarajan, R. P., Choi, S., Hong, C., He, D., Pekmezci, M., et al. (2015) · Science · doi:10.1126/science.aab0015 · Cited by 566
Making sense of G‐quadruplex and i‐motif functions in oncogene promoters
Brooks, T. A., Kendrick, S., & Hurley, L. (2010) · The FEBS Journal · doi:10.1111/j.1742-4658.2010.07759.x · Cited by 477
Self-citation
The role of supercoiling in transcriptional control of MYC and its importance in molecular therapeutics
Brooks, T. A., & Hurley, L. H. (2009) · Nature Reviews Cancer · doi:10.1038/nrc2733 · Cited by 294
Self-citation
mRNA decay factor AUF1 maintains normal aging, telomere maintenance, and suppression of senescence by activation of telomerase transcription
Pont, A. R., Sadri, N., Hsiao, S. J., Smith, S., & Schneider, R. J. (2012) · Molecular Cell · doi:10.1016/j.molcel.2012.04.019 · Cited by 139

See all 279

Authors: Palumbo, S. L., Ebbinghaus, S. W., & Hurley, L. H.
Venue: Journal of the American Chemical Society
Publisher: American Chemical Society (ACS)
Published: 2009-07-14
Volume: 131
Issue: 31
Pages: 10878-10891
ISSN: 0002-7863 (ISSN-L)
1520-5126
DOI: 10.1021/ja902281d
OpenAlex: W2022287291
Cited by: 279
References: 60
FWCI: 5.76
Type: journal article
Language: en
Open access: green

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.

Formation of a Unique End-to-End Stacked Pair of G-Quadruplexes in the hTERT Core Promoter with Implications for Inhibition of Telomerase by G-Quadruplex-Interactive Ligands

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Self-check

Citations

Cites

Cited by

Metadata

Record

About these checks

Data sources