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Genetic Incorporation of a Small, Environmentally Sensitive, Fluorescent Probe into Proteins in Saccharomyces cerevisiae
Open accessAbstract
Here, we report that the fluorescent amino acid, 3-(6-acetylnaphthalen-2-ylamino)-2-aminopropanoic acid (Anap), can be genetically incorporated into proteins in yeast with excellent selectivity and efficiency by means of an orthogonal tRNA/aminoacyl-tRNA synthetase pair. This small, environmentally sensitive fluorophore was site-specifically incorporated into Escherichia coli glutamine binding protein and used to directly probe local structural changes caused by ligand binding. The small size of Anap and the ability to introduce it by simple mutagenesis at defined sites in the proteome make it a useful local probe of protein structure, molecular interactions, protein folding, and localization.
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Cited by
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Liu, C. C., & Schultz, P. G. (2010) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.052308.105824 · Cited by 1,725
Self-citationCellular Incorporation of Unnatural Amino Acids and Bioorthogonal Labeling of Proteins
Lang, K., & Chin, J. W. (2014) · Chemical Reviews · doi:10.1021/cr400355w · Cited by 1,035
Expanding and Reprogramming the Genetic Code of Cells and Animals
Chin, J. W. (2014) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-060713-035737 · Cited by 476
A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging
Specht, E. A., Braselmann, E., & Palmer, A. E. (2017) · Annual Review of Physiology · doi:10.1146/annurev-physiol-022516-034055 · Cited by 444
Beyond the Canonical 20 Amino Acids: Expanding the Genetic Lexicon
Young, T. S., & Schultz, P. G. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.r109.091306 · Cited by 354
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Metadata
- Authors
- Lee, H. S., Guo, J., Lemke, E. A., Dimla, R. D., & Schultz, P. G.
- Venue
- Journal of the American Chemical Society
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-08-24
- Volume
- 131
- Issue
- 36
- Pages
- 12921-12923
- ISSN
- 0002-7863 (ISSN-L)
- 1520-5126
- OpenAlex
- W1964078197
- Cited by
- 226
- References
- 0
- FWCI
- 3.48
- Type
- journal article
- Language
- en
- Open access
- green
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- Lee, H. S., Guo, J., Lemke, E. A., Dimla, R. D., & Schultz, P. G. (2009). Genetic Incorporation of a Small, Environmentally Sensitive, Fluorescent Probe into Proteins in Saccharomyces cerevisiae. Journal of the American Chemical Society. https://doi.org/10.1021/ja904896s
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