Quantification of Exopolysaccharide, Lactic Acid, and Lactose Concentrations in Culture Broth by near-Infrared Spectroscopy

Macedo, Maria G.; Laporte, Marie-France; Lacroix, Christophe

doi:10.1021/jf0110093

Cited by 36Spectroscopy and Chemometric AnalysesSpectroscopy Techniques in Biomedical and Chemical ResearchProbiotics and Fermented Foods

Quantification of Exopolysaccharide, Lactic Acid, and Lactose Concentrations in Culture Broth by near-Infrared Spectroscopy

Macedo, Maria G., Laporte, Marie-France, Lacroix, Christophe·2002·Journal of Agricultural and Food Chemistry

DOI 10.1021/jf0110093

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Abstract

Near-infrared spectroscopy (NIRS) was used for the simultaneous prediction of exopolysaccharide (EPS; 0-3 g/L) and lactic acid (0-59 g/L) productions as well as lactose (0-68 g/L) concentration in supernatant samples from pH-controlled batch cultures of Lactobacillus rhamnosus RW-9595M in supplemented whey permeate medium. To develop calibration equations, the correlation between the second derivative of 164 NIRS transmittance spectra and concentration data obtained with reference methods was calculated at the wavelength between 1653-1770 and 2041-2353 nm, using a partial least-squares method (PLS). The lactic acid and lactose concentrations were measured by HPLC, and the EPS concentration was estimated by a new ultrafiltration method. The PLS correlation coefficient (R(2)) and the standard error of cross-validation for the calibrations were 91% and 0.26 g/L for EPS, 99% and 2.54 g/L for lactic acid, and 98% and 3.32 g/L for lactose, respectively. The calibration equations were validated with 45 randomly selected culture samples from 6 cultures that were not used for calibration. A high agreement between data of the reference methods and those of NIRS was observed, with correlation coefficients and standard errors of prediction of 99% and 1.64 g/L for lactic acid, 99% and 4.5 g/L for lactose, and 91% and 0.32 g/L for EPS. The results suggest that NIRS could be a useful method for rapid monitoring and control of EPS lactic fermentations.

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A near infrared spectroscopy method for rapid and simultaneous quantification of lactic acid, lactose and EPS directly in culture media without prior isolation led to results with strong correlation (R2 > 0.90) with reference methods.
“For a rapid and simultaneous quantification of lactic acid, lactose and EPS directly in culture media without a prior isolation step, a near infrared (NIR) spectroscopy method has also been reported by Macedo et al. [29] This method led to results with strong correlation (R2 > 0.90) with reference methods to indicate the effectiveness for monitoring the production of EPS and lactic acid during fer”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [29]

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Authors: Macedo, M. G., Laporte, M.-F., & Lacroix, C.
Affiliations: Université Laval (CA)
Corresponding author: Lacroix, Christophe
ORCID: Lacroix, Christophe
Venue: Journal of Agricultural and Food Chemistry
Publisher: American Chemical Society (ACS)
Published: 2002-03-01
Volume: 50
Issue: 7
Pages: 1774-1779
ISSN: 0021-8561 (ISSN-L)
1520-5118
DOI: 10.1021/jf0110093
OpenAlex: W2013821852
Cited by: 36
References: 20
Citations by year: 2025: 3 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 3 · 2016: 3 · 2015: 3 · 2014: 3
FWCI: 1.90
Type: journal article
Language: en
Open access: closed
Locations: Journal of Agricultural and Food Chemistry (publishedVersion)
PubMed (publishedVersion)
Topics: Physical Sciences › Chemistry › Analytical Chemistry › Spectroscopy and Chemometric Analyses
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords: exopolysaccharides, lactic acid, lactose, Lactobacillus rhamnosus, whey permeate, near-infrared spectroscopy, partial least squares calibration, second derivative spectra, fermentation monitoring, batch culture
MeSH terms: Chromatography, High Pressure Liquid, Hydrogen-Ion Concentration, Lactobacillus, Lactose, Polysaccharides, Bacterial, Culture Media, Conditioned, Spectroscopy, Near-Infrared, Lactic Acid

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Quantification of Exopolysaccharide, Lactic Acid, and Lactose Concentrations in Culture Broth by near-Infrared Spectroscopy

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