The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Novel Fluorometric Assay for Hydroxyl Radical Prevention Capacity Using Fluorescein as the Probe
Abstract
A novel fluorometric method has been developed to evaluate hydroxyl radical prevention capacity using fluorescein (FL) as the probe. The hydroxyl radical is generated by a Co(II)-mediated Fenton-like reaction, and the hydroxyl radical formation under the experimental condition is indirectly confirmed by the hydroxylation of p-hydroxybenzoic acid. The fluorescence decay curve of FL is monitored in the absence or presence of antioxidant, the area under the fluorescence decay curve (AUC) is integrated, and the net AUC, which is an index of the hydroxyl radical prevention capacity, is calculated by subtracting the AUC of the blank from that of the antioxidant. Gallic acid is chosen as a reference standard, and the activity of sample is expressed as gallic acid equivalents. The method is rigorously validated through linearity, precision, accuracy, and ruggedness. A wide range of phenolic antioxidants is analyzed, and the hydroxyl radical prevention capacity is mainly due to the metal-chelating capability of the compounds.
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Cites
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Oxidants, oxidative stress and the biology of ageing
Finkel, T., & Holbrook, N. J. (2000) · Nature · doi:10.1038/35041687 · Cited by 9,413
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Interaction of Iron with Polyphenolic Compounds: Application to Antioxidant Characterization
Yoshino, M., & Murakami, K. (1998) · Analytical Biochemistry · doi:10.1006/abio.1997.2522 · Cited by 291
Comments on the Mechanism of the “Fenton-Like” Reaction
Goldstein, S., & Meyerstein, D. (1999) · Accounts of Chemical Research · doi:10.1021/ar9800789 · Cited by 210
Hydrogen Peroxide. Ubiquitous in Cell Culture and In vivo?
Halliwell, B., Clement, M. V., Ramalingam, J., & Long, L. H. (2000) · IUBMB Life · doi:10.1080/15216540051080930 · Cited by 196
Cited by
The Chemistry behind Antioxidant Capacity Assays
Huang, D., Ou, B., & Prior, R. L. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf030723c · Cited by 6,162
Self-citationAntioxidants and antioxidant methods: an updated overview
Gulcin, İ. (2020) · Archives of Toxicology · doi:10.1007/s00204-020-02689-3 · Cited by 2,205
Fluorescence probes used for detection of reactive oxygen species
Gomes, A., Fernandes, E., & Lima, J. L. F. C. (2005) · Journal of Biochemical and Biophysical Methods · doi:10.1016/j.jbbm.2005.10.003 · Cited by 1,844
Environmental Implications of Hydroxyl Radicals (•OH)
Gligorovski, S., Strekowski, R., Barbati, S., & Vione, D. (2015) · Chemical Reviews · doi:10.1021/cr500310b · Cited by 1,623
Review of methods to determine antioxidant capacities
Karadag, A., Ozcelik, B., & Saner, S. (2009) · Food Analytical Methods · doi:10.1007/s12161-008-9067-7 · Cited by 753
Metadata
- Authors
- Ou, B., Hampsch-Woodill, M., Flanagan, J., Deemer, E. K., Prior, R. L., & Huang, D.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-04-05
- Volume
- 50
- Issue
- 10
- Pages
- 2772-2777
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1985091566
- Cited by
- 349
- References
- 7
- FWCI
- 2.17
- Type
- journal article
- Language
- en
- Open access
- closed
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