Extraction, semi-quantification, and fast on-line identification of oligopeptides in Grana Padano cheese by HPLC-MS.
Abstract
Sixteen samples of Grana Padano cheese aged from 2 to 33 months were analyzed by HPLC-MS. The extraction process involved the use of diluted HCl, thus avoiding a strong deproteinizing agent (TCA), and allowing to maintain in solution also very lipophilic peptides. The molecular mass of the most abundant peptides were determined by electrospray ionization (ESI) mass spectrometry. A new method was developed based on the small fragmentation peaks arising from in-source fragmentation and from software analysis of the known casein sequences, which in many cases allowed the direct on-line identification of the oligopeptide sequences. Several new peptides never previously reported were identified, some of which containing bioactive sequences, consistently with what was described in the literature. Semiquantification of peptides at the different stages of aging was also performed by using a suitable internal standard, providing new insights into the evolution of the oligopeptide fraction during aging.
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The third cluster mainly contains phosphopeptides, and all of them derive from the region 11-28 of β-casein.
“The third cluster mainly contains phosphopeptides, and all of them derive from the region 11-28 of β-casein, in agreement with previous work (Sforza et al., 2003).”
From The Interrelationship Between Microbiota and Peptides During Ripening as a Driver for Parmigiano Reggiano Cheese Quality (2020) · cites this paper as Sforza et al., 2003
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- Authors
- Sforza, S., Ferroni, L., Galaverna, G., Dossena, A., & Marchelli, R.
- Affiliations
- University of Parma (IT)
- Corresponding author
- Marchelli, Rosangela
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-03-07
- Volume
- 51
- Issue
- 8
- Pages
- 2130-2135
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2073140469
- Cited by
- 46
- References
- 15
- Citations by year
- 2025: 2 · 2021: 3 · 2020: 3 · 2018: 2 · 2017: 3 · 2016: 4 · 2015: 1 · 2014: 4 · 2012: 7
- FWCI
- 1.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- Grana Padano cheese, oligopeptides, cheese aging, in-source fragmentation, peptide identification, peptide extraction, HCl extraction, bioactive peptides, internal standard, electrospray ionization mass spectrometry
- MeSH terms
- Cheese, Chromatography, High Pressure Liquid, Molecular Weight, Oligopeptides, Time Factors, Spectrometry, Mass, Electrospray Ionization
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