Uncommonly thorough hydrolysis of peptides during ripening of Ragusano cheese revealed by tandem mass spectrometry.
Open accessAbstract
Ragusano is a pasta filata cheese produced from raw milk in Sicily. The proteolysis was extensively analyzed after stretching (day 0), at 4 and 7 months of ripening through soluble nitrogen, urea-PAGE, and peptide identification by tandem mass spectrometry. After stretching, 123 peptides were identified: 72 arising from β-casein, 34 from α(s1)-casein, and 17 from α(s2)-casein. The main protein splitting corresponded to the action of plasmin, chymosin, cathepsin D, cell envelope proteinase, and peptidase activities of lactic acid bacteria. Unlike other types of cheeses, <10% residual β- and α(s)-caseins remained intact at 7 months, indicating original network organization based on large casein fragments. The number of identified soluble peptides also dramatically decreased after 4 and 7 months of ripening, to 47 and 25, respectively. Among them, bioactive peptides were found, that is, mineral carrier, antihypertensive, and immunomodulating peptides and phosphopeptides.
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Bottari, B., Levante, A., Bancalari, E., Sforza, S., Bottesini, C., Prandi, B., De Filippis, F., Ercolini, D., Nocetti, M., & Gatti, M. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.581658 · Cited by 41 · see citing works
- Authors
- Gagnaire, V., Carpino, S., Pediliggieri, C., Jardin, J., Lortal, S., & Licitra, G.
- Affiliations
- Institut Agro Rennes-Angers (FR)
- CoRFiLaC, 25 Km 5, Ragusa Mare, 97100 Ragusa, Italy
- University of Catania (IT)
- Corresponding author
- Gagnaire, Valérie
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-11-10
- Volume
- 59
- Issue
- 23
- Pages
- 12443-12452
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2317683676
- Cited by
- 31
- References
- 48
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 6 · 2015: 1 · 2014: 2 · 2012: 1
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- green
- hal.science
- Licence
- other-oa
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- pasta filata cheese, cheese ripening, proteolysis, peptide hydrolysis, β-casein, αs1-casein, αs2-casein, plasmin, chymosin, cathepsin D, bioactive peptides, antihypertensive peptides, immunomodulating peptides, phosphopeptides, raw milk cheese
- MeSH terms
- Amino Acid Sequence, Caseins, Cheese, Chromatography, High Pressure Liquid, Food Handling, Hydrolysis, Molecular Sequence Data, Peptide Hydrolases, Peptides, Tandem Mass Spectrometry, Chemical Phenomena
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