Cited by 31Protein Hydrolysis and Bioactive PeptidesProteins in Food SystemsFood and Agricultural Sciences

Uncommonly thorough hydrolysis of peptides during ripening of Ragusano cheese revealed by tandem mass spectrometry.

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Gagnaire, Valérie, Carpino, Stefania, Pediliggieri, Concetta, Jardin, Julien, Lortal, Sylvie, Licitra, Giuseppe·2011·Journal of Agricultural and Food Chemistry

Abstract

Ragusano is a pasta filata cheese produced from raw milk in Sicily. The proteolysis was extensively analyzed after stretching (day 0), at 4 and 7 months of ripening through soluble nitrogen, urea-PAGE, and peptide identification by tandem mass spectrometry. After stretching, 123 peptides were identified: 72 arising from β-casein, 34 from α(s1)-casein, and 17 from α(s2)-casein. The main protein splitting corresponded to the action of plasmin, chymosin, cathepsin D, cell envelope proteinase, and peptidase activities of lactic acid bacteria. Unlike other types of cheeses, <10% residual β- and α(s)-caseins remained intact at 7 months, indicating original network organization based on large casein fragments. The number of identified soluble peptides also dramatically decreased after 4 and 7 months of ripening, to 47 and 25, respectively. Among them, bioactive peptides were found, that is, mineral carrier, antihypertensive, and immunomodulating peptides and phosphopeptides.

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Crossref · 10.1021/jf2027268

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Authors
Gagnaire, V., Carpino, S., Pediliggieri, C., Jardin, J., Lortal, S., & Licitra, G.
Affiliations
  • Institut Agro Rennes-Angers (FR)
  • CoRFiLaC, 25 Km 5, Ragusa Mare, 97100 Ragusa, Italy
  • University of Catania (IT)
Corresponding author
Gagnaire, Valérie
Venue
Journal of Agricultural and Food Chemistry
Published
2011-11-10
Volume
59
Issue
23
Pages
12443-12452
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2317683676
Cited by
31
References
48
Citations by year
2026: 5 · 2025: 2 · 2024: 2 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 2 · 2018: 1 · 2017: 2 · 2016: 6 · 2015: 1 · 2014: 2 · 2012: 1
FWCI
0.39
Type
journal article
Language
en
Open access
Licence
other-oa
Locations
  • Journal of Agricultural and Food Chemistry (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Proteins in Food Systems
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
Keywords
pasta filata cheese, cheese ripening, proteolysis, peptide hydrolysis, β-casein, αs1-casein, αs2-casein, plasmin, chymosin, cathepsin D, bioactive peptides, antihypertensive peptides, immunomodulating peptides, phosphopeptides, raw milk cheese
MeSH terms
Amino Acid Sequence, Caseins, Cheese, Chromatography, High Pressure Liquid, Food Handling, Hydrolysis, Molecular Sequence Data, Peptide Hydrolases, Peptides, Tandem Mass Spectrometry, Chemical Phenomena

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