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Phenolics Composition and Antioxidant Activity of Sweet Basil (Ocimum basilicum L.)
Abstract
The antioxidant activity of a methanolic extract of Ocimum basilicum L. (sweet basil) was examined using different in vitro assay model systems. The crude extract was fractionated on a Sephadex LH-20 column, and six fractions were identified. The DPPH scavenging assay system and the oxidation of the soy phosphotidylcholin liposome model system were used to evaluate the antioxidant activity of each fraction. Fraction IV showed the strongest activity followed by fractions V and VI. Phenolic compounds responsible for the antioxidative activity of the fractions were characterized by atmospheric pressure chemical ionization liquid chromatography-mass spectrometry. The major antioxidant compound in fraction IV was confirmed as rosmarinic acid by (1)H NMR and characteristic fragmentations in the mass spectrum. Moreover, the native of antioxidant activity of rosmarinic acid in the liposome system was examined. The results showed that one rosmarinic acid can capture 1.52 radicals, and furthermore, the existence of a synergistic effect between alpha-tocopherol and rosmarinic acid was revealed.
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Cited by
Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet
Viuda‐Martos, M., Ruiz Navajas, Y., Sánchez Zapata, E., Fernández‐López, J., & Pérez‐Álvarez, J. A. (2010) · Flavour and Fragrance Journal · doi:10.1002/ffj.1951 · Cited by 338
Majdi, C., Pereira, C., Dias, M. I., Calhelha, R. C., Alves, M. J., Rhourri-Frih, B., Charrouf, Z., Barros, L., Amaral, J. S., & Ferreira, I. C. F. R. (2020) · Antioxidants · doi:10.3390/antiox9050369 · Cited by 88
Park, J. B. (2011) · International Journal of Food Sciences and Nutrition · doi:10.3109/09637486.2011.562882 · Cited by 88
Singh, V., Kahol, A., Singh, I. P., Saraf, I., & Shri, R. (2016) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2016.10.026 · Cited by 64
Singh, V., Kaur, K., Kaur, S., Shri, R., Singh, T. G., & Singh, M. (2022) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2022.115438 · Cited by 40
Metadata
- Authors
- Jayasinghe, C., Gotoh, N., Aoki, T., & Wada, S.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2003-06-06
- Volume
- 51
- Issue
- 15
- Pages
- 4442-4449
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1976603846
- Cited by
- 308
- References
- 0
- FWCI
- 2.95
- Type
- journal article
- Language
- en
- Open access
- closed
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- Jayasinghe, C., Gotoh, N., Aoki, T., & Wada, S. (2003). Phenolics Composition and Antioxidant Activity of Sweet Basil (Ocimum basilicum L.). Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf034269o
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