CoChat
Sign Up
Cited by 40Medicinal Plants and NeuroprotectionPhytochemicals and Medicinal PlantsFlavonoids in Medical Research

Trimethoxyflavones from Ocimum basilicum L. leaves improve long term memory in mice by modulating multiple pathways

Singh, Varinder, Kaur, Kiranpreet, Kaur, Sanimardeep, Shri, Richa, Singh, Thakur Gurjeet, Singh, Manjinder·2022·Journal of Ethnopharmacology
DOI 10.1016/j.jep.2022.115438
Open this paper in CoChat — free account

Abstract

Ethnopharmacological relevance Traditionally, Ocimum basilicum L. leaves (OB) are recommended for various brain disorders.Aim of the study Scientific evidence highlights the cognition improvement capacity of Ocimum basilicum L. leave extract (OBE), however, the compound(s) responsible for this effect and the associated mechanism was not reported. The present study was, thus, designed to isolate and identify the compound responsible for memory improvement effects of OB and to delineate the associated mechanism of action.Materials and methods In-vitro acetylcholinesterase (AChE) inhibitory (Ellman method) and antioxidant (DPPH scavenging) assays guided fractionation was employed to isolate the bioactive compounds from OBE. The isolated compounds were characterised using spectroscopic techniques (FTIR, NMR and MS). In-silico and in-vivo [mouse model of scopolamine (SCOP) induced amnesia] investigations were used to substantiate the memory improvement effects of isolated compounds and to understand their mechanism of action.Results AChE and DPPH assays guided fractionation of OBE lead to isolation of two pure compounds namely, 5,7-dihydroxy-3',4',5'-trimethoxyflavone (S1) and 3-hydroxy-3',4',5'-trimethoxyflavone (S2). Both S1 and S2 mitigated the cognitive impairment due to SCOP in mice by reducing brain AChE activity, TBARS, TNF-α, IL-1β, IL-6 and caspase-3 concentrations and elevating reduced glutathione and IL-10 levels; together with amelioration of brain hippocampus histopathological aberration (H and E staining). Moreover, the molecular docking of S1 and S2 at the active pockets of AChE and caspase-3 has shown good interactions with vital amino acid residues.Conclusions Our findings show that trimethoxy flavones are responsible for the memory improvement effect of OBE due to their anticholinergic, antioxidant, anti-inflammatory and anti-apoptotic properties. These maybe developed as valuable alternatives for management of cognitive disorders.

Integrity signals

Registry

Crossref · 10.1016/j.jep.2022.115438

Cites

72 of 72 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Extracts from Ocimum basilicum, Lavandula stoechas, Schisandra chinensis, and Ginkgo biloba had neuroprotective effects with significant reductions in AChE activity (p<0.001) and improvements in memory and oxidative stress markers.

    “Further investigations into extracts from Ocimum basilicum, Lavandula stoechas, Schisandra chinensis, and Ginkgo biloba have highlighted their neuroprotective effects, with significant reductions in AChE activity (p < 0.001) and improvements in memory and oxidative stress markers [49,50,51,52,53].”

    From Cholinesterase Inhibitors from Plants and Their Potential in Alzheimer’s Treatment: Systematic Review (2025) · cites this paper as [49]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Singh, V., Kaur, K., Kaur, S., Shri, R., Singh, T. G., & Singh, M.
Affiliations
  • Chitkara University (IN)
  • Punjabi University (IN)
Corresponding author
  • Singh, Varinder
  • Shri, Richa
ORCID
Venue
Journal of Ethnopharmacology
Publisher
Elsevier BV
Published
2022-09
Volume
295
Pages
115438
ISSN
0378-8741 (ISSN-L)
DOI
10.1016/j.jep.2022.115438
OpenAlex
W4281907524
Cited by
40
References
72
Citations by year
2026: 8 · 2025: 17 · 2024: 7 · 2023: 8
FWCI
8.33
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Journal of Ethnopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Complementary and alternative medicine › Medicinal Plants and Neuroprotection
  • Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
  • Health Sciences › Medicine › Pharmacology › Flavonoids in Medical Research
Keywords
Ocimum basilicum, long-term memory, scopolamine-induced amnesia, acetylcholinesterase inhibition, antioxidant activity, anti-inflammatory activity, anti-apoptotic effects, hippocampus, cognitive impairment, neuroprotection, caspase-3, TNF-α, IL-10, glutathione, flavonoid isolation
MeSH terms
Acetylcholinesterase, Animals, Antioxidants, Scopolamine, Plant Leaves, Ocimum basilicum, Mice, Caspase 3, Memory, Long-Term, Molecular Docking Simulation

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review