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Antimicrobial and Antioxidant Effects of Milk Protein-Based Film Containing Essential Oils for the Preservation of Whole Beef Muscle

Oussalah, Mounia, Caillet, Stéphane, Salmiéri, Stéphane, Saucier, Linda, Lacroix, Monique·2004·Journal of Agricultural and Food Chemistry

Abstract

Milk protein-based edible films containing 1.0% (w/v) oregano, 1.0% (w/v) pimento, or 1.0% oregano-pimento (1:1) essential oils mix were applied on beef muscle slices to control the growth of pathogenic bacteria and increase the shelf life during storage at 4 degrees C. Meat and film were periodically tested during 7 days for microbial and biochemical analysis. The lipid oxidation potential of meat was evaluated by the determination of thiobarbituric reactive substances (TBARS). The availability of phenolic compounds from essential oils was evaluated by the determination of total phenolic compounds present in the films during storage. Antioxidant properties of films during storage were also evaluated following a modified procedure of the N,N-diethyl-p-phenylenediamine colorimetric method. Oregano-based films stabilized lipid oxidation in beef muscle samples, whereas pimento-based films presented the highest antioxidant activity. The application of bioactive films on meat surfaces containing 10(3) colony-forming units/cm2 of Escherichia coli O157:H7 or Pseudomonas spp. showed that film containing oregano was the most effective against both bacteria, whereas film containing pimento oils seems to be the least effective against these two bacteria. A 0.95 log reduction of Pseudomonas spp. level, as compared to samples without film, was observed at the end of storage in the presence of films containing oregano extracts. A 1.12 log reduction of E. coli O157:H7 level was noted in samples coated with oregano-based films.

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Metadata

Authors
Oussalah, M., Caillet, S., Salmiéri, S., Saucier, L., & Lacroix, M.
Venue
Journal of Agricultural and Food Chemistry
Published
2004-08-06
Volume
52
Issue
18
Pages
5598-5605
ISSN
  • 0021-8561 (ISSN-L)
  • 1520-5118
OpenAlex
W2006793260
Cited by
407
References
1
FWCI
5.24
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Antimicrobial and Antioxidant Effects of Milk Protein-Based Film Containing Essential Oils for the Preservation of Whole Beef Muscle. https://app.cochat.ai/papers/10.1021/jf049389q
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Oussalah, M., Caillet, S., Salmiéri, S., Saucier, L., & Lacroix, M. (2004). Antimicrobial and Antioxidant Effects of Milk Protein-Based Film Containing Essential Oils for the Preservation of Whole Beef Muscle. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf049389q

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