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High-Throughput Relative DPPH Radical Scavenging Capacity Assay
Abstract
A high-throughput relative 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging capacity (RDSC) assay was developed and validated in the present study. This RDSC assay is easy to perform and has acceptable accuracy (90-110% recovery), precision [3.9-7.0% pooled relative standard deviation (RSD)], and reproducibility (2.2 and 3.5% interday and intraday RSD). This assay reports the RDSC values for antioxidant samples, which make it possible to compare the DPPH radical scavenging capacities of antioxidants determined in different laboratories. The RDSC assay may be conducted in aqueous alcohol and acetone for hydrophilic antioxidants or in the organic solvents for lipophilic antioxidants without solubilizing agents, which makes it possible to directly compare the radical scavenging capacities of hydrophilic and lipophilic antioxidants. In addition, the high-throughput RDSC assay could be utilized for EC50 value estimation. The high-throughput RDSC assay may be used for screening and investigating potential natural antioxidants.
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Vegetables, Fruit, and Cancer Prevention
STEINMETZ, K. A., & POTTER, J. D. (1996) · Journal of the American Dietetic Association · doi:10.1016/s0002-8223(96)00273-8 · Cited by 2,118
Review of methods to determine chain-breaking antioxidant activity in food
ROGINSKY, V, & LISSI, E. (2005) · Food Chemistry · doi:10.1016/j.foodchem.2004.08.004 · Cited by 855
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Analytical Methods Used in Determining Antioxidant Activity: A Review
Munteanu, I. G., & Apetrei, C. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22073380 · Cited by 2,119
Antioxidant Compounds and Their Antioxidant Mechanism
Francenia Santos-Sánchez, N., Salas-Coronado, R., Villanueva-Cañongo, C., & Hernández-Carlos, B. (2019) · Antioxidants · doi:10.5772/intechopen.85270 · Cited by 594
Buranasudja, V., Rani, D., Malla, A., Kobtrakul, K., & Vimolmangkang, S. (2021) · Scientific Reports · doi:10.1038/s41598-021-92958-7 · Cited by 112
Modified DPPH and ABTS Assays to Assess the Antioxidant Profile of Untreated Oils
Christodouleas, D. C., Fotakis, C., Nikokavoura, A., Papadopoulos, K., & Calokerinos, A. C. (2015) · Food Analytical Methods · doi:10.1007/s12161-014-0005-6 · Cited by 95
Malafatti, J. O. D., da Costa Brito, S., Pereira, V. A. C., Calisto, Í., Netto, S. B., Formigoni, C. E., Innocentini, M. D. D. M., Mitsuyuki, M. C., Sivieri, K., & Ferreira, M. D. (2025) · Packaging Technology and Science · doi:10.1002/pts.2880
Metadata
- Authors
- Cheng, Z., Moore, J., & Yu, L. (.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2006-09-07
- Volume
- 54
- Issue
- 20
- Pages
- 7429-7436
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2060093563
- Cited by
- 405
- References
- 2
- FWCI
- 4.73
- Type
- journal article
- Language
- en
- Open access
- closed
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