The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Cellular Antioxidant Activity (CAA) Assay for Assessing Antioxidants, Foods, and Dietary Supplements
Abstract
A cellular antioxidant activity (CAA) assay for quantifying the antioxidant activity of phytochemicals, food extracts, and dietary supplements has been developed. Dichlorofluorescin is a probe that is trapped within cells and is easily oxidized to fluorescent dichlorofluorescein (DCF). The method measures the ability of compounds to prevent the formation of DCF by 2,2'-azobis(2-amidinopropane) dihydrochloride (ABAP)-generated peroxyl radicals in human hepatocarcinoma HepG2 cells. The decrease in cellular fluorescence when compared to the control cells indicates the antioxidant capacity of the compounds. The antioxidant activities of selected phytochemicals and fruit extracts were evaluated using the CAA assay, and the results were expressed in micromoles of quercetin equivalents per 100 micromol of phytochemical or micromoles of quercetin equivalents per 100 g of fresh fruit. Quercetin had the highest CAA value, followed by kaempferol, epigallocatechin gallate (EGCG), myricetin, and luteolin among the pure compounds tested. Among the selected fruits tested, blueberry had the highest CAA value, followed by cranberry > apple = red grape > green grape. The CAA assay is a more biologically relevant method than the popular chemistry antioxidant activity assays because it accounts for some aspects of uptake, metabolism, and location of antioxidant compounds within cells.
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Cites
The Chemistry behind Antioxidant Capacity Assays
Huang, D., Ou, B., & Prior, R. L. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf030723c · Cited by 6,162
Vegetables, Fruit, and Cancer Prevention
STEINMETZ, K. A., & POTTER, J. D. (1996) · Journal of the American Dietetic Association · doi:10.1016/s0002-8223(96)00273-8 · Cited by 2,118
Antioxidant activity of apple peels
Wolfe, K., Wu, X., & Liu, R. H. (2003) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020782a · Cited by 1,873
Self-citationThe Effect of Fruit and Vegetable Intake on Risk for Coronary Heart Disease
Joshipura, K. J., Hu, F. B., Manson, J. E., Stampfer, M. J., Rimm, E. B., Speizer, F. E., Colditz, G., Ascherio, A., Rosner, B., Spiegelman, D., et al. (2001) · Annals of Internal Medicine · doi:10.7326/0003-4819-134-12-200106190-00010 · Cited by 1,396
Fruit and vegetable intake and risk of major chronic disease
Hung, H.-C., Joshipura, K. J., Jiang, R., Hu, F. B., Hunter, D., Smith-Warner, S. A., Colditz, G. A., Rosner, B., Spiegelman, D., & Willett, W. C. (2004) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/djh296 · Cited by 1,327
Antioxidant and Antiproliferative Activities of Common Vegetables
Chu, Y.-F., Sun, J., Wu, X., & Liu, R. H. (2002) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf020665f · Cited by 937
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Dietary polyphenols, oxidative stress and antioxidant and anti-inflammatory effects
Zhang, H., & Tsao, R. (2016) · Current Opinion in Food Science · doi:10.1016/j.cofs.2016.02.002 · Cited by 1,519
Review of methods to determine antioxidant capacities
Karadag, A., Ozcelik, B., & Saner, S. (2009) · Food Analytical Methods · doi:10.1007/s12161-008-9067-7 · Cited by 753
Phenolic compounds in fruits – an overview
Haminiuk, C. W. I., Maciel, G. M., Plata‐Oviedo, M. S. V., & Peralta, R. M. (2012) · International Journal of Food Science & Technology · doi:10.1111/j.1365-2621.2012.03067.x · Cited by 566
Carotenoids and Chlorophylls as Antioxidants
Pérez-Gálvez, A., Viera, I., & Roca, M. (2020) · Antioxidants · doi:10.3390/antiox9060505 · Cited by 519
Xiong, Y., Zhang, P., Warner, R. D., & Fang, Z. (2019) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12506 · Cited by 336
Metadata
- Authors
- Wolfe, K. L., & Liu, R. H.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2007-09-29
- Volume
- 55
- Issue
- 22
- Pages
- 8896-8907
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1986112503
- Cited by
- 1,219
- References
- 6
- FWCI
- 12.41
- Type
- journal article
- Language
- en
- Open access
- closed
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