The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives
Abstract
The impact of lignins of various origins on filter paper hydrolysis by fungal cellulase was evaluated. Powdered lignins were added to enzyme incubations, either as isolated or after thorough hydroxypropylation of phenolic sites. Extent of cellulose hydrolysis was reduced by 14−60% by the addition of up to 15% lignin to the substrate. Unmodified lignins were more detrimental to cellulose hydrolysis than hydroxypropylated lignins. The inhibitory effect of lignin addition was only partially overcome by a 10-fold increase in cellulase activity, suggesting inhibitory lignin interactions with both substrate and enzyme. Preincubation of cellulase with underivatized lignins resulted in reduced enzyme activity and soluble protein concentration in the supernatant, suggesting protein precipitation with lignin rather than reduced activity of a lignin−enzyme complex as the inhibitory mechanism. Two further experiments showed that the negative impact of lignin on cellulose hydrolysis can be counteracted by addition of various N compounds and by ammoniation. Keywords: Lignin; cellulose degradation; cellulase inhibition; free phenolic group; ammoniation; PEG; PVP; N compounds
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Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(91)78551-2 · Cited by 28,658
Cited by
Pretreatment: the key to unlocking low‐cost cellulosic ethanol
Yang, B., & Wyman, C. E. (2008) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.49 · Cited by 1,524
Enzymatic conversion of lignocellulose into fermentable sugars: challenges and opportunities
Jørgensen, H., Kristensen, J. B., & Felby, C. (2007) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.4 · Cited by 1,143
Van Dyk, J. S., & Pletschke, B. I. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.03.002 · Cited by 1,015
Zhao, X., Zhang, L., & Liu, D. (2012) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.1331 · Cited by 986
Grabber, J. H. (2005) · Crop Science · doi:10.2135/cropsci2004.0191 · Cited by 574
Metadata
- Authors
- Sewalt, V. J. H., Glasser, W. G., & Beauchemin, K. A.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-05-01
- Volume
- 45
- Issue
- 5
- Pages
- 1823-1828
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2084912368
- Cited by
- 273
- References
- 1
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
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