The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Protein Hydrolysates from Pacific Whiting Solid Wastes
Abstract
Alcalase and Neutrase showed optimum activity against Pacific whiting solid wastes (PWSW) at pH 9.5, 60 °C and pH 7.0, 55 °C, respectively. Alcalase had a higher proteolytic activity than Neutrase. Enzyme concentration, reaction time, and waste/buffer ratio significantly affected the hydrolysis and nitrogen recovery (NR) ( p < 0.05). Optimum conditions for PWSW hydrolysis were 20 AU Alcalase/kg, 1 h reaction time, waste/buffer ratio of 1:1 (w/v). Correlation between the degree of hydrolysis (DH) and NR ( R 2 = 0.970−0.978) was high. Freeze-dried hydrolysate was brownish yellow in color ( L * = 54.59, a * = 6.70, b * = 27.89) and contained 2.77% moisture, 79.97% protein, 13.44% ash, and 3.83% lipid. Amino acid composition of freeze-dried hydrolysate was similar to that of PWSW and Pacific whiting muscle but tryptophan was reduced to 21.50% and 14.74%, respectively. Keywords: Waste; Pacific whiting; hydrolysate; Alcalase; Neutrase
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Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Cited by
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Brooks MS, R. V. (2013) · Journal of Microbial & Biochemical Technology · doi:10.4172/1948-5948.1000110 · Cited by 376
Enzymatic hydrolysis of proteins from yellowfin tuna (Thunnus albacares) wastes using Alcalase
Guérard, F., Dufossé, L., De La Broise, D., & Binet, A. (2001) · Journal of Molecular Catalysis B: Enzymatic · doi:10.1016/s1381-1177(00)00031-x · Cited by 246
Girgih, A. T., Udenigwe, C. C., Li, H., Adebiyi, A. P., & Aluko, R. E. (2011) · Journal of the American Oil Chemists' Society · doi:10.1007/s11746-011-1841-9 · Cited by 174
Extraction of protein from food waste: An overview of current status and opportunities
Kamal, H., Le, C. F., Salter, A. M., & Ali, A. (2021) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12739 · Cited by 170
Adhikari, B., Chae, M., & Bressler, D. (2018) · Polymers · doi:10.3390/polym10020176 · Cited by 132
Metadata
- Authors
- Benjakul, S., & Morrissey, M. T.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1997-09-01
- Volume
- 45
- Issue
- 9
- Pages
- 3423-3430
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2053624808
- Cited by
- 581
- References
- 1
- FWCI
- 0.68
- Type
- journal article
- Language
- en
- Open access
- closed
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