The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
PCR−RFLP Analysis of Mitochondrial DNA: A Reliable Method for Species Identification
Abstract
A method for identification of game species has been developed on the basis of the amplification of a specific part of the mitochondrial genome (tRNA(Glu)/cytochrome b) using the polymerase chain reaction (PCR). To distinguish between several game species, the obtained 464-bp-long PCR products were cut with different restriction endonucleases (RE) resulting in species-specific restriction fragment length polymorphism (RFLP). Even closely related deer species could be distinguished by application of one or two RE. Natural polymorphisms of the target sequence within one species were examined for red deer (Cervus elaphus), and base pair substitutions were identified affecting the RFLP pattern.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Food authentication by PCR-based methods
Mafra, I., Ferreira, I. M. P. L. V. O., & Oliveira, M. B. P. P. (2008) · European Food Research and Technology · doi:10.1007/s00217-007-0782-x · Cited by 372
Metadata
- Authors
- Wolf, C., Rentsch, J., & Hübner, P.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-04-01
- Volume
- 47
- Issue
- 4
- Pages
- 1350-1355
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2033657932
- Cited by
- 232
- References
- 1
- FWCI
- 1.30
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: PCR−RFLP Analysis of Mitochondrial DNA: A Reliable Method for Species Identification. https://app.cochat.ai/papers/10.1021/jf9808426
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- Wolf, C., Rentsch, J., & Hübner, P. (1999). PCR−RFLP Analysis of Mitochondrial DNA: A Reliable Method for Species Identification. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf9808426
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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