Purification and Identification of Components of γ-Oryzanol in Rice Bran Oil
Abstract
High-purity gamma-oryzanol was obtained from crude rice bran oil using a normal-phase preparative scale HPLC. A reverse-phase HPLC method was used for separating the individual components of gamma-oryzanol present in rice bran oil. Ten fractions were isolated and collected using the reverse-phase HPLC method, and their structures were identified. Identification was accomplished using GC/MS with an electron impact mass spectrum after components were transformed into trimethylsilyl ether derivatives. The 10 components of gamma-oryzanol were identified as Delta(7)-stigmastenyl ferulate, stigmasteryl ferulate, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, Delta(7)-campestenyl ferulate, campesteryl ferulate, Delta(7)-sitostenyl ferulate, sitosteryl ferulate, compestanyl ferulate, and sitostanyl ferulate. Three of these, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, and campesteryl ferulate, were major components of gamma-oryzanol.
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Rice proteins and peptides, obtained through enzymatic hydrolysis of rice bran or germ, exhibit bioactivities beneficial for skin care.
“Rice proteins and peptides, obtained through enzymatic hydrolysis of rice bran or germ, exhibit bioactivities beneficial for skin care.”
From Pharmacognostic and dermatological insights into Oryza sativa: A comprehensive review of its skin benefits and bioactive compounds (2025) · cites this paper as Xu and Godber, 1999
Rice pastes made by grinding cooked rice are applied topically to soothe skin irritations, sunburns, and minor wounds, capitalizing on rice's natural anti-inflammatory and healing effects.
“In some Asian cultures, rice pastes made by grinding cooked rice are applied topically to soothe skin irritations, sunburns, and minor wounds, capitalizing on rice's natural anti-inflammatory and healing effects (Sun et al., 2016;Xu and Godber, 1999;Han et al., 2019;Kim and Lee, 2017).”
From Pharmacognostic and dermatological insights into Oryza sativa: A comprehensive review of its skin benefits and bioactive compounds (2025) · cites this paper as Xu and Godber, 1999
Rice water has been used as a gentle facial cleanser and skin toner due to its mild exfoliating, moisturizing, and soothing properties.
“Rice water has been used as a gentle facial cleanser and skin toner due to its mild exfoliating, moisturizing, and soothing properties.”
From Pharmacognostic and dermatological insights into Oryza sativa: A comprehensive review of its skin benefits and bioactive compounds (2025) · cites this paper as Xu and Godber, 1999
Certain peptides derived from rice proteins possess antioxidant and anti-inflammatory properties, contributing to skin rejuvenation and protection against environmental stressors.
“Certain peptides derived from rice proteins have also been shown to possess antioxidant and anti-inflammatory properties, contributing to skin rejuvenation and protection against environmental stressors (Sun et al., 2016;Xu and Godber, 1999).”
From Pharmacognostic and dermatological insights into Oryza sativa: A comprehensive review of its skin benefits and bioactive compounds (2025) · cites this paper as Xu and Godber, 1999
Rice proteins and peptides can act as moisturizers by improving water retention and skin elasticity.
“They can act as moisturizers by improving water retention and skin elasticity.”
From Pharmacognostic and dermatological insights into Oryza sativa: A comprehensive review of its skin benefits and bioactive compounds (2025) · cites this paper as Xu and Godber, 1999
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Mubassir, Naseem, A., Km., P., & Km., N. B. (2025) · Journal of Pharmacognosy and Phytotherapy · doi:10.5897/jpp2025.0647
- Authors
- Xu, Z., & Godber, J. S.
- Affiliations
- Louisiana State University (US)
- ORCID
- Xu, Zhimin
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-06-09
- Volume
- 47
- Issue
- 7
- Pages
- 2724-2728
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2077577005
- Cited by
- 368
- References
- 0
- Citations by year
- 2026: 4 · 2025: 12 · 2024: 9 · 2023: 15 · 2022: 15 · 2021: 14 · 2020: 23 · 2019: 15 · 2018: 18 · 2017: 7 · 2016: 10 · 2015: 17 · 2014: 25 · 2013: 15 · 2012: 34
- FWCI
- 2.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Civil War Book Review (submittedVersion)
- Topics
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Physical Sciences › Chemistry › Organic Chemistry › Edible Oils Quality and Analysis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Keywords
- γ-oryzanol, rice bran oil, stigmasterol, campesterol, cycloartenol, 24-methylenecycloartanol, structural identification, GC-MS identification
- MeSH terms
- Rice Bran Oil, Chromatography, High Pressure Liquid, Phenylpropionates, Plant Oils
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