Antioxidant effectiveness as influenced by phenolic content of fresh orange juices
Abstract
Several fresh orange juices, obtained from five different Citrus sinensis (L.) Osbeck varieties (three pigmented varieties, Moro, Sanguinello, and Tarocco, and two blond varieties, Valencia late and Washington navel), were subjected to antioxidant profile determination (including total polyphenols, flavanones, anthocyanins, hydroxycinnamic acids, and ascorbic acid). The antioxidant activity of these juices was then assessed by means of different "in vitro" tests (bleaching of the stable 1,1-diphenyl-2-picrylhydrazyl radical; peroxidation, induced by the water-soluble radical initiator 2,2'-azobis(2-amidinopropane) hydrochloride, on mixed dipalmitoylphosphatidylcholine/linoleic acid unilamellar vesicles; scavenging activity against nitric oxide; total antioxidant status). All orange juices tested showed an evident antioxidant effect. Our findings indicate the following: (1) the antioxidant efficiency of orange juices may be attributed, in a significant part at least, to their content of total phenols, (2) while ascorbic acid seems to play a minor role; (3) the antioxidant activity of orange juices is related not only to structural features of phytochemicals contained in them, but also to their capability to interact with biomembranes; (4) finally, as to pigmented juices, their antioxidant efficiency appears to be widely influenced by the anthocyanin level. One could speculate that the supply of natural antioxidant phenols through daily consumption of orange juice might provide…
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Genotype and extrinsic stressors affect the formation of bioactive compounds.
“as declared by Rapisarda et al. [67] and Rouphael et al. [68], it is the genotype and the extrinsic stressors that affect the formation of bioactive compounds”
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- Authors
- Rapisarda, P., Tomaino, A., Lo Cascio, R., Bonina, F., De Pasquale, A., & Saija, A.
- Affiliations
- University of Messina (IT)
- Istituto Sperimentale per l'Agrumicoltura, Acireale (CT), Italy, Department Farmaco-Biologico, University of Messina, Contrada Annunziata, 98168 Messina, Italy, Department of Pharmaceutical Sciences, University of Catania, Catania, Italy, and Department of Pharmacology of Natural Substances and General Physiology, University “La Sapienza” of Rome, Rome, Italy
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-10-02
- Volume
- 47
- Issue
- 11
- Pages
- 4718-4723
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2042203812
- Cited by
- 380
- References
- 1
- Citations by year
- 2026: 3 · 2025: 11 · 2024: 6 · 2023: 5 · 2022: 13 · 2021: 13 · 2020: 14 · 2019: 12 · 2018: 10 · 2017: 14 · 2016: 16 · 2015: 21 · 2014: 19 · 2013: 23 · 2012: 24
- FWCI
- 4.74
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- orange juice, Citrus sinensis, antioxidant activity, total polyphenols, flavanones, anthocyanins, hydroxycinnamic acids, vitamin C, DPPH radical scavenging, lipid peroxidation, nitric oxide scavenging, total antioxidant capacity, Moro, Valencia Late, Washington navel, natural antioxidants
- MeSH terms
- Antioxidants, Beverages, Free Radicals, Fruit, Phenols, Regression Analysis
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