The DOI matches its Crossref record; 16 of the 16 references it cites were found in a registry or an index.
Flavonols from Saffron Flower: Tyrosinase Inhibitory Activity and Inhibition Mechanism
Abstract
A common flavonol, kaempferol, isolated from the fresh flower petals of Crocus sativus L. (Iridaceae) was found to inhibit the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom tyrosinase with an ID(50) of 67 microgram/mL (0.23 mM). Interestingly, its 3-O-glycoside derivatives did not inhibit this oxidation. The inhibition kinetics analyzed by a Lineweaver-Burk plot found kaempferol to be a competitive inhibitor, and this inhibitory activity presumably comes from its ability to chelate copper in the enzyme. This copper chelation mechanism can be applicable for all of the flavonols as long as their 3-hydroxyl group is free. However, quercetin, kaempferol, and galangin each affect the oxidation of L-tyrosine in somewhat different ways.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The Systematic Identification of Flavonoids
Mabry, T. J., Markham, K. R., & Thomas, M. B. (1970) · Springer eBooks · doi:10.1007/978-3-642-88458-0 · Cited by 3,920
The antioxidants of higher plants
Larson, R. A. (1988) · Phytochemistry · doi:10.1016/0031-9422(88)80254-1 · Cited by 2,007
Tyrosinase: a comprehensive review of its mechanism
Sánchez-Ferrer, Á., Neptuno Rodríguez-López, J., García-Cánovas, F., & García-Carmona, F. (1995) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/0167-4838(94)00204-t · Cited by 1,239
Jovanovic, S. V., Steenken, S., Tosic, M., Marjanovic, B., & Simic, M. G. (1994) · Journal of the American Chemical Society · doi:10.1021/ja00090a032 · Cited by 1,194
Antioxidant activity of flavonoids and reactivity with peroxy radical
Torel, J., Cillard, J., & Cillard, P. (1986) · Phytochemistry · doi:10.1016/s0031-9422(00)85485-0 · Cited by 602
Cabanes, J., Chazarra, S., & Garcia-Carmona, F. (1994) · Journal of Pharmacy and Pharmacology · doi:10.1111/j.2042-7158.1994.tb03253.x · Cited by 453
Metal Ions in Biological Systems
Österberg, R. (1980) · Bioenergetics and Thermodynamics: Model Systems · doi:10.1007/978-94-009-9035-7_33 · Cited by 350
Substrate analog binding to the coupled binuclear copper active site in tyrosinase
Wilcox, D. E., Porras, A. G., Hwang, Y. T., Lerch, K., Winkler, M. E., & Solomon, E. I. (1985) · Journal of the American Chemical Society · doi:10.1021/ja00299a043 · Cited by 311
Stability of flavonoid complexes of copper(II) and flavonoid antioxidant activity
Thompson, M., Williams, C. R., & Elliot, G. E. P. (1976) · Analytica Chimica Acta · doi:10.1016/s0003-2670(01)84703-6 · Cited by 183
Kader, F., Rovel, B., Girardin, M., & Metche, M. (1996) · Food Chemistry · doi:10.1016/0308-8146(95)00068-2 · Cited by 141
Cited by
A comprehensive review on tyrosinase inhibitors
Zolghadri, S., Bahrami, A., Hassan Khan, M. T., Munoz-Munoz, J., Garcia-Molina, F., Garcia-Canovas, F., & Saboury, A. A. (2019) · Journal of Enzyme Inhibition and Medicinal Chemistry · doi:10.1080/14756366.2018.1545767 · Cited by 1,078
Hypopigmenting agents: an updated review on biological, chemical and clinical aspects
Solano, F., Briganti, S., Picardo, M., & Ghanem, G. (2006) · Pigment Cell Research · doi:10.1111/j.1600-0749.2006.00334.x · Cited by 696
Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials
Abdullaev, F. I., & Espinosa-Aguirre, J. J. (2004) · Cancer Detection and Prevention · doi:10.1016/j.cdp.2004.09.002 · Cited by 434
Survey and mechanism of skin depigmenting and lightening agents
Parvez, S., Kang, M., Chung, H., Cho, C., Hong, M., Shin, M., & Bae, H. (2006) · Phytotherapy Research · doi:10.1002/ptr.1954 · Cited by 403
Polyphenol-Rich Purple Corn Pericarp Extract Adversely Impacts Herbivore Growth and Development
Tayal, M., Somavat, P., Rodriguez, I., Thomas, T., Christoffersen, B., & Kariyat, R. (2020) · Insects · doi:10.3390/insects11020098 · Cited by 56
Metadata
- Authors
- Kubo, I., & Kinst-Hori, I.
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-09-16
- Volume
- 47
- Issue
- 10
- Pages
- 4121-4125
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W1975966519
- Cited by
- 274
- References
- 16
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Flavonols from Saffron Flower: Tyrosinase Inhibitory Activity and Inhibition Mechanism. https://app.cochat.ai/papers/10.1021/jf990201q
- The paper
- Kubo, I., & Kinst-Hori, I. (1999). Flavonols from Saffron Flower: Tyrosinase Inhibitory Activity and Inhibition Mechanism. Journal of Agricultural and Food Chemistry. https://doi.org/10.1021/jf990201q
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
