Extraction of Azadirachtin A from Neem Seed Kernels by Supercritical Fluid and Its Evaluation by HPLC and LC/MS
Open accessAbstract
A new supercritical extraction methodology was applied to extract azadirachtin A (AZA-A) from neem seed kernels. Supercritical and liquid carbon dioxide (CO(2)) were used as extractive agents in a three-separation-stage supercritical pilot plant. Subcritical conditions were tested too. Comparisons were carried out by calculating the efficiency of the pilot plant with respect to the milligrams per kilogram of seeds (ms/mo) of AZA-A extracted. The most convenient extraction was gained using an ms/mo ratio of 119 rather than 64. For supercritical extraction, a separation of cuticular waxes from oil was set up in the pilot plant. HPLC and electrospray mass spectroscopy were used to monitor the yield of AZA-A extraction.
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- Authors
- Ambrosino, P., Fresa, R., Fogliano, V., Monti, S. M., & Ritieni, A.
- Affiliations
- University of Salerno (IT)
- Venue
- Journal of Agricultural and Food Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 1999-11-25
- Volume
- 47
- Issue
- 12
- Pages
- 5252-5256
- ISSN
- 0021-8561 (ISSN-L)
- 1520-5118
- OpenAlex
- W2087874152
- Semantic Scholar
- d4707466b467665b2321f84129ac66e831e45591
- Cited by
- 44
- References
- 9
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 5 · 2019: 1 · 2017: 1 · 2016: 1 · 2014: 2 · 2013: 1 · 2012: 3
- FWCI
- 0.71
- Type
- journal article
- Language
- en
- Open access
- green
- research.wur.nl
- Locations
- Journal of Agricultural and Food Chemistry (publishedVersion)
- PubMed (publishedVersion)
- Socio-Environmental Systems Modeling (acceptedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Funders
- U.S. Food and Drug Administration
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Phase Equilibria and Thermodynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical compounds biological activities
- Keywords
- azadirachtin A, neem seed kernel, supercritical fluid extraction, liquid carbon dioxide, subcritical extraction, extraction efficiency, cuticular wax, oil extraction
- MeSH terms
- Animals, Chromatography, High Pressure Liquid, Insecticides, Gas Chromatography-Mass Spectrometry, Seeds, Trees, Triterpenes, Limonins
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