The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Fully 2‘-Modified Oligonucleotide Duplexes with Improved in Vitro Potency and Stability Compared to Unmodified Small Interfering RNA
Abstract
We have identified a small interfering RNA (siRNA) motif, consisting entirely of 2'-O-methyl and 2'-fluoro nucleotides, that displays enhanced plasma stability and increased in vitro potency. At one site, this motif showed remarkable >500-fold improvement in potency over the unmodified siRNA. This marks the first report of such a potent fully modified motif, which may represent a useful design for therapeutic oligonucleotides.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
1 retracted
Correction: Retraction: Unaltered cosmic spherules in a 1.4-Gyr-old sandstone from Finland
Deutsch, A., Greshake, A., PESONEN, L. J., & Pihlaja, P. (2004) · Nature · doi:10.1038/nature02607 · Cited by 5
RetractedDuplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., & Tuschl, T. (2001) · Nature · doi:10.1038/35078107 · Cited by 9,456
An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
Hammond, S. M., Bernstein, E., Beach, D., & Hannon, G. J. (2000) · Nature · doi:10.1038/35005107 · Cited by 3,003
Asymmetry in the Assembly of the RNAi Enzyme Complex
Schwarz, D. S., Hutvágner, G., Du, T., Xu, Z., Aronin, N., & Zamore, P. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00759-1 · Cited by 2,922
Argonaute2 is the catalytic engine of mammalian RNAi
Liu, J., Carmell, M. A., Rivas, F. V., Marsden, C. G., Thomson, J. M., Song, J.-J., Hammond, S. M., Joshua-Tor, L., & Hannon, G. J. (2004) · Science · doi:10.1126/science.1102513 · Cited by 2,796
Functional siRNAs and miRNAs exhibit strand bias
Khvorova, A., Reynolds, A., & Jayasena, S. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00801-8 · Cited by 2,645
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
Soutschek, J., Akinc, A., Bramlage, B., Charisse, K., Constien, R., Donoghue, M., Elbashir, S., Geick, A., Hadwiger, P., Harborth, J., et al. (2004) · Nature · doi:10.1038/nature03121 · Cited by 2,183
Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi
Martinez, J., Patkaniowska, A., Urlaub, H., Lührmann, R., & Tuschl, T. (2002) · Cell · doi:10.1016/s0092-8674(02)00908-x · Cited by 1,459
RNA interference targeting Fas protects mice from fulminant hepatitis
Song, E., Lee, S.-K., Wang, J., Ince, N., Ouyang, N., Min, J., Chen, J., Shankar, P., & Lieberman, J. (2003) · Nature Medicine · doi:10.1038/nm828 · Cited by 1,110
Caplen, N. J., Parrish, S., Imani, F., Fire, A., & Morgan, R. A. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.171251798 · Cited by 1,043
ATP Requirements and Small Interfering RNA Structure in the RNA Interference Pathway
Nykänen, A., Haley, B., & Zamore, P. D. (2001) · Cell · doi:10.1016/s0092-8674(01)00547-5 · Cited by 1,016
siRNA function in RNAi: A chemical modification analysis
CHIU, Y.-L., & RANA, T. M. (2003) · RNA · doi:10.1261/rna.5103703 · Cited by 860
Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain
Ma, J.-B., Ye, K., & Patel, D. J. (2004) · Nature · doi:10.1038/nature02519 · Cited by 732
Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells
Czauderna, F. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg393 · Cited by 623
In vivo activity of nuclease-resistant siRNAs
LAYZER, J. M., MCCAFFREY, A. P., TANNER, A. K., HUANG, Z., KAY, M. A., & SULLENGER, B. A. (2004) · RNA · doi:10.1261/rna.5239604 · Cited by 580
Song, J.-J., Liu, J., Tolia, N. H., Schneiderman, J., Smith, S. K., Martienssen, R. A., Hannon, G. J., & Joshua-Tor, L. (2003) · Nature Structural & Molecular Biology · doi:10.1038/nsb1016 · Cited by 575
Kinetic analysis of the RNAi enzyme complex
Haley, B., & Zamore, P. D. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb780 · Cited by 544
Tolerance for mutations and chemical modifications in a siRNA
Amarzguioui, M. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg147 · Cited by 534
RNA Interference in Mammalian Cells by Chemically-Modified RNA
Braasch, D. A., Jensen, S., Liu, Y., Kaur, K., Arar, K., White, M. A., & Corey, D. R. (2003) · Biochemistry · doi:10.1021/bi0343774 · Cited by 531
Structure and conserved RNA binding of the PAZ domain
Yan, K. S., Yan, S., Farooq, A., Han, A., Zeng, L., & Zhou, M.-M. (2003) · Nature · doi:10.1038/nature02129 · Cited by 488
Harborth, J., Elbashir, S. M., Vandenburgh, K., Manninga, H., Scaringe, S. A., Weber, K., & Tuschl, T. (2003) · Antisense and Nucleic Acid Drug Development · doi:10.1089/108729003321629638 · Cited by 474
Evidence that siRNAs Function as Guides, Not Primers, in the Drosophila and Human RNAi Pathways
Schwarz, D. S., Hutvágner, G., Haley, B., & Zamore, P. D. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00651-2 · Cited by 471
Gene Silencing by Systemic Delivery of Synthetic siRNAs in Adult Mice
Sørensen, D. R., Leirdal, M., & Sioud, M. (2003) · Journal of Molecular Biology · doi:10.1016/s0022-2836(03)00181-5 · Cited by 464
Efficient Reduction of Target RNAs by Small Interfering RNA and RNase H-dependent Antisense Agents
Vickers, T. A., Koo, S., Bennett, C. F., Crooke, S. T., Dean, N. M., & Baker, B. F. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m210326200 · Cited by 461
Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain
Lingel, A., Simon, B., Izaurralde, E., & Sattler, M. (2003) · Nature · doi:10.1038/nature02123 · Cited by 450
Kawasaki, A. M., Casper, M. D., Freier, S. M., Lesnik, E. A., Zounes, M. C., Cummins, L. L., Gonzalez, C., & Cook, P. D. (1993) · Journal of Medicinal Chemistry · doi:10.1021/jm00059a007 · Cited by 438
Caspase 8 small interfering RNA prevents acute liver failure in mice
Zender, L., Hütker, S., Liedtke, C., Tillmann, H. L., Zender, S., Mundt, B., Waltemathe, M., Gösling, T., Flemming, P., Malek, N. P., et al. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1330920100 · Cited by 384
Molecular mechanisms of action of antisense drugs
Crooke, S. T. (1999) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/s0167-4781(99)00148-7 · Cited by 358
Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain
Lingel, A., Simon, B., Izaurralde, E., & Sattler, M. (2004) · Nature Structural & Molecular Biology · doi:10.1038/nsmb777 · Cited by 349
Inhibition of HIV-1 Infection by Small Interfering RNA-Mediated RNA Interference
Capodici, J., Karikó, K., & Weissman, D. (2002) · The Journal of Immunology · doi:10.4049/jimmunol.169.9.5196 · Cited by 302
Biodistribution of phosphodiester and phosphorothioate siRNA
Braasch, D. A., Paroo, Z., Constantinescu, A., Ren, G., Öz, O. K., Mason, R. P., & Corey, D. R. (2004) · Bioorganic & Medicinal Chemistry Letters · doi:10.1016/j.bmcl.2003.12.074 · Cited by 251
Zhang, X., Shan, P., Jiang, D., Noble, P. W., Abraham, N. G., Kappas, A., & Lee, P. J. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m312941200 · Cited by 241
A genetic link between co-suppression and RNA interference in C. elegans
Ketting, R. F., & Plasterk, R. H. A. (2000) · Nature · doi:10.1038/35005113 · Cited by 214
Functional siRNAs and miRNAs Exhibit Strand Bias
Khvorova, A., Reynolds, A., & Jayasena, S. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00893-6 · Cited by 210
Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide
Polacek, N., Gaynor, M., Yassin, A., & Mankin, A. S. (2001) · Nature · doi:10.1038/35078113 · Cited by 205
Quantitation of Phosphorothioate Oligonucleotides in Human Plasma
Leeds, J. M., Graham, M. J., Truong, L., & Cummins, L. L. (1996) · Analytical Biochemistry · doi:10.1006/abio.1996.0088 · Cited by 143
Holen, T., Amarzguioui, M., Babaie, E., & Prydz, H. (2003) · Nucleic Acids Research · doi:10.1093/nar/gkg338 · Cited by 123
Hashimoto, J. G., Beadles-Bohling, A. S., & Wiren, K. M. (2004) · BioTechniques · doi:10.2144/04361bm06 · Cited by 114
Cited by
The current state and future directions of RNAi-based therapeutics
Setten, R. L., Rossi, J. J., & Han, S.-P. (2019) · Nature Reviews Drug Discovery · doi:10.1038/s41573-019-0017-4 · Cited by 1,443
Bennett, C. F., & Swayze, E. E. (2010) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pharmtox.010909.105654 · Cited by 1,374
Self-citationThe chemical evolution of oligonucleotide therapies of clinical utility
Khvorova, A., & Watts, J. K. (2017) · Nature Biotechnology · doi:10.1038/nbt.3765 · Cited by 1,216
Interfering with disease: a progress report on siRNA-based therapeutics
de Fougerolles, A., Vornlocher, H.-P., Maraganore, J., & Lieberman, J. (2007) · Nature Reviews Drug Discovery · doi:10.1038/nrd2310 · Cited by 1,203
RNAi therapeutics: a potential new class of pharmaceutical drugs
Bumcrot, D., Manoharan, M., Koteliansky, V., & Sah, D. W. Y. (2006) · Nature Chemical Biology · doi:10.1038/nchembio839 · Cited by 1,085
Metadata
- Authors
- Allerson, C. R., Sioufi, N., Jarres, R., Prakash, T. P., Naik, N., Berdeja, A., Wanders, L., Griffey, R. H., Swayze, E. E., & Bhat, B.
- Venue
- Journal of Medicinal Chemistry
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-01-20
- Volume
- 48
- Issue
- 4
- Pages
- 901-904
- ISSN
- 0022-2623 (ISSN-L)
- 1520-4804
- OpenAlex
- W2107762071
- Cited by
- 448
- References
- 38
- FWCI
- 9.22
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
