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Asymmetry in the Assembly of the RNAi Enzyme Complex

Schwarz, Dianne S., Hutvágner, György, Du, Tingting, Xu, Zuoshang, Aronin, Neil, Zamore, Phillip D.·2003·Cell
DOI 10.1016/s0092-8674(03)00759-1
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  • The strand in which the 5' end is less tightly paired to its complement remains associated with Ago and performs the functions of the mature miRNA.

    “Studies investigating the relative thermodynamic stability of the two ends of the pre-miRNA duplex indicated that the strand in which the 5Ј end is less tightly paired to its complement remains associated with Ago and performs the functions of the mature miRNA (Khvorova et al., 2003;Schwarz et al., 2003).”

    From Comprehensive expression analyses of neural cell-type-specific miRNAs identify new determinants of the specification and maintenance of neuronal phenotypes (2013) · cites this paper as Schwarz et al., 2003

  • The first four nucleotides of each end of the duplex are believed to establish thermodynamic asymmetry, and the difference of a single extra hydrogen bond is sufficient to drive preferential loading of the less stable strand in vitro.

    “The first four nucleotides of each end of the duplex are believed to establish thermodynamic asymmetry, and the difference of a single extra hydrogen bond is sufficient to drive preferential loading of the less stable strand in vitro (Khvorova, Reynolds, & Jayasena, 2003; Krol et al., 2004; Schwarz et al., 2003).”

    From microRNA strand selection: Unwinding the rules (2021) · cites this paper as Schwarz et al., 2003

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Cited by

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Authors
Schwarz, D. S., Hutvágner, G., Du, T., Xu, Z., Aronin, N., & Zamore, P. D.
Venue
Cell
Publisher
Elsevier BV
Published
2003-10
Volume
115
Issue
2
Pages
199-208
ISSN
0092-8674
DOI
10.1016/s0092-8674(03)00759-1
Cited by
2,323
References
2
Language
en
Licence

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