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Poly(ethylene glycol) Hydrogel Microstructures Encapsulating Living Cells
Abstract
We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 micrometers in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 micrometers allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.
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Cited by
Hydrogels in Biology and Medicine: From Molecular Principles to Bionanotechnology
Peppas, N. A., Hilt, J. Z., Khademhosseini, A., & Langer, R. (2006) · Advanced Materials · doi:10.1002/adma.200501612 · Cited by 4,109
Hydrogels in regenerative medicine
Slaughter, B. V., Khurshid, S. S., Fisher, O. Z., Khademhosseini, A., & Peppas, N. A. (2009) · Advanced Materials · doi:10.1002/adma.200802106 · Cited by 2,703
Cell Encapsulation in Biodegradable Hydrogels for Tissue Engineering Applications
Nicodemus, G. D., & Bryant, S. J. (2008) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2007.0332 · Cited by 1,239
Biomaterials for bone tissue engineering
Stevens, M. M. (2008) · Materials Today · doi:10.1016/s1369-7021(08)70086-5 · Cited by 1,202
Patterning surfaces with functional polymers
Nie, Z., & Kumacheva, E. (2008) · Nature Materials · doi:10.1038/nmat2109 · Cited by 935
Metadata
- Authors
- Koh, W.-G., Revzin, A., & Pishko, M. V.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-02-21
- Volume
- 18
- Issue
- 7
- Pages
- 2459-2462
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2058242764
- Cited by
- 232
- References
- 0
- FWCI
- 4.17
- Type
- journal article
- Language
- en
- Open access
- closed
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- Koh, W.-G., Revzin, A., & Pishko, M. V. (2002). Poly(ethylene glycol) Hydrogel Microstructures Encapsulating Living Cells. Langmuir. https://doi.org/10.1021/la0115740
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