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Poly(ethylene glycol) Hydrogel Microstructures Encapsulating Living Cells

Koh, Won-Gun, Revzin, Alexander, Pishko, Michael V.·2002·Langmuir

Abstract

We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 micrometers in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 micrometers allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.

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Metadata

Authors
Koh, W.-G., Revzin, A., & Pishko, M. V.
Venue
Langmuir
Published
2002-02-21
Volume
18
Issue
7
Pages
2459-2462
ISSN
  • 0743-7463 (ISSN-L)
  • 1520-5827
OpenAlex
W2058242764
Cited by
232
References
0
FWCI
4.17
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Poly(ethylene glycol) Hydrogel Microstructures Encapsulating Living Cells. https://app.cochat.ai/papers/10.1021/la0115740
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Koh, W.-G., Revzin, A., & Pishko, M. V. (2002). Poly(ethylene glycol) Hydrogel Microstructures Encapsulating Living Cells. Langmuir. https://doi.org/10.1021/la0115740

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