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Probing the Orientation of Surface-Immobilized Immunoglobulin G by Time-of-Flight Secondary Ion Mass Spectrometry
Abstract
Static time-of-flight secondary ion mass spectrometry (ToF-SIMS) is a powerful surface analysis technique for the characterization of protein films because of its chemical selectivity and surface sensitivity. In this study, static ToF-SIMS and principal component analysis (PCA), a multivariate data analysis method, were combined to probe the orientation of surface-immobilized immunoglobulin G (IgG). IgG orientation can enhance its ability to detect its antigen in immunoassay techniques. The IgG used in this work is the mouse monoclonal anti-human chorionic gonadotropin (anti-hCG). Anti-hCG films on different well-defined substrates have been studied using its F(ab')2 and Fc fragments as references. Atomic force microscopy was used to characterize these protein films before static ToF-SIMS analysis. The results from PCA of ToF-SIMS spectra were related to the antibody primary amino acid composition and its three-dimensional structure.
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Cited by
Mediation of biomaterial-cell interactions by adsorbed proteins: a review
Wilson, C. J., Clegg, R. E., Leavesley, D. I., & Pearcy, M. J. (2005) · Tissue Engineering · doi:10.1089/ten.2005.11.1 · Cited by 1,696
Orientation and characterization of immobilized antibodies for improved immunoassays (Review)
Welch, N. G., Scoble, J. A., Muir, B. W., & Pigram, P. J. (2017) · Biointerphases · doi:10.1116/1.4978435 · Cited by 414
Influence of PEG Architecture on Protein Adsorption and Conformation
Michel, R., Pasche, S., Textor, M., & Castner, D. G. (2005) · Langmuir · doi:10.1021/la051726h · Cited by 340
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Metadata
- Authors
- Wang, H., Castner, D. G., Ratner, B. D., & Jiang, S.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-01-31
- Volume
- 20
- Issue
- 5
- Pages
- 1877-1887
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2032325457
- Cited by
- 163
- References
- 0
- FWCI
- 10.78
- Type
- journal article
- Language
- en
- Open access
- closed
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- Wang, H., Castner, D. G., Ratner, B. D., & Jiang, S. (2004). Probing the Orientation of Surface-Immobilized Immunoglobulin G by Time-of-Flight Secondary Ion Mass Spectrometry. Langmuir. https://doi.org/10.1021/la035376f
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