Role of nonadsorbing polymers in bacterial aggregation
Abstract
Bacteria exhibit properties similar to those of nonbiological colloids and can display pairwise attractions when in close proximity. This interaction is governed by the surface chemistry of the cells. We seek to understand bacterial aggregation at the cellular level using Escherichia coli (E. coli) AB1157. Aggregation studies were carried out using 0.5 to 2.5 wt% E. coli AB1157 harvested in different growth phases with varying concentrations of a nonadsorbing polymer, sodium polystyrene sulfonate (SPS). The electrophoretic mobility of E. coli AB1157 in different growth phases was determined using phase-amplitude light scattering. E. coli AB1157 was found to be negatively charged, and the cell surface properties changed in different growth phases. The electrokinetic results correlated well with the different concentrations of nonadsorbing polymer needed to induce depletion aggregation. This shows that a difference in aggregation properties is due to changes in the bacteria electrokinetic properties during their growth.
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Non-adsorbing polymers may cause bacteria to autoaggregate through depletion interactions.
“Further, non-adsorbing polymers may cause bacteria to autoaggregate through depletion interactions [82].”
From Bacterial autoaggregation (2018) · cites this paper as [82]
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Trunk, T., Bacterial Cell Surface Group, S. F. G. A. E. B., S. Khalil, H., & C. Leo, J. (2018) · AIMS Microbiology · doi:10.3934/microbiol.2018.1.140 · Cited by 444 · see citing works
“Further, non-adsorbing polymers may cause bacteria to autoaggregate through depletion interactions [82].”
BackgroundIsenring, J., Geirnaert, A., Lacroix, C., & Stevens, M. J. A. (2021) · BMC Microbiology · doi:10.1186/s12866-021-02331-x · Cited by 42 · see citing works
- Authors
- Eboigbodin, K. E., Newton, J. R. A., Routh, A. F., & Biggs, C. A.
- Affiliations
- University of Sheffield (GB)
- Corresponding author
- Biggs, C. A.
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2005-11-11
- Volume
- 21
- Issue
- 26
- Pages
- 12315-12319
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W1995795423
- Cited by
- 62
- References
- 48
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 4 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 2 · 2014: 1 · 2013: 1 · 2012: 2
- FWCI
- 2.38
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Langmuir (publishedVersion)
- PubMed (publishedVersion)
- SHURA (Sheffield Hallam University Research Archive) (Sheffield Hallam University) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/C505391/1)
- Topics
- Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Electrostatics and Colloid Interactions
- Physical Sciences › Environmental Science › Water Science and Technology › Coagulation and Flocculation Studies
- Physical Sciences › Physics and Astronomy › Condensed Matter Physics › Micro and Nano Robotics
- Keywords
- bacterial aggregation, non-adsorbing polymers, sodium polystyrene sulfonate, Escherichia coli, electrophoretic mobility, bacterial surface charge, growth phase, colloidal interactions
- MeSH terms
- Adsorption, Bacterial Adhesion, Electrophoresis, Escherichia coli, Polymers
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