Factors Affecting Exocellular Polysaccharide Production by Lactobacillus delbrueckii subsp.bulgaricus Grown in a Chemically Defined Medium
Open accessAbstract
We developed a chemically defined medium (CDM) containing lactose or glucose as the carbon source that supports growth and exopolysaccharide (EPS) production of two strains of Lactobacillus delbrueckii subsp. bulgaricus. The factors found to affect EPS production in this medium were oxygen, pH, temperature, and medium constituents, such as orotic acid and the carbon source. EPS production was greatest during the stationary phase. Composition analysis of EPS isolated at different growth phases and produced under different fermentation conditions (varying carbon source or pH) revealed that the component sugars were the same. The EPS from strain L. delbrueckii subsp. bulgaricus CNRZ 1187 contained galactose and glucose, and that of strain L. delbrueckii subsp. bulgaricus CNRZ 416 contained galactose, glucose, and rhamnose. However, the relative proportions of the individual monosaccharides differed, suggesting that repeating unit structures can vary according to specific medium alterations. Under pH-controlled fermentation conditions, L. delbrueckii subsp. bulgaricus strains produced as much EPS in the CDM as in milk. Furthermore, the relative proportions of individual monosaccharides of EPS produced in pH-controlled CDM or in milk were very similar. The CDM we developed may be a useful model and an alternative to milk in studies of EPS production.
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Lcb. casei and Lb. delbreuckii subsp. bulgaricus strains cultured in fermented milk produced a lower amount of EPS (<600 mg/L) compared to M17 broth supplemented with different carbon and nitrogen sources (1500 mg/L).
“On the other hand, Lcb. casei and Lb. delbreuckii subsp. bulgaricus strains cultured in fermented milk produced a lower amount of EPS (<600 mg/L) compared to M17 broth supplemented with different carbon and nitrogen sources (1500 mg/L) [60].”
From Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods (2021) · cites this paper as [60]
The introduction reports EPS yields of 50–425 mg/L in milk, supporting the claim that milk yields were below 600 mg/L. But the paper studies L. delbrueckii subsp. bulgaricus in a chemically defined medium, not L. casei in M17 broth, and does not report the claimed 1,500 mg/L M17 yield. The Results instead say that, with pH control, yields in the defined medium were about the same as in milk. (LLM, conf=0.88)
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- Authors
- Petry, S., Furlan, S., Crepeau, M.-J., Cerning, J., & Desmazeaud, M.
- Affiliations
- <!--label omitted: 1-->Unité de Recherches Laitières et Génétique Appliquée, INRA, 78352 Jouy-en-Josas Cedex,1 and
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- <!--label omitted: 2-->Unité de Recherche sur les Polysaccharides, leur Organisation et Interaction, INRA, 44316 Nantes Cedex,2 France
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-08
- Volume
- 66
- Issue
- 8
- Pages
- 3427-3431
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC92166
- OpenAlex
- W2144916775
- Cited by
- 209
- References
- 16
- Citations by year
- 2026: 6 · 2025: 4 · 2024: 8 · 2023: 10 · 2022: 13 · 2021: 8 · 2020: 9 · 2019: 11 · 2018: 7 · 2017: 11 · 2016: 5 · 2015: 13 · 2014: 15 · 2013: 7 · 2012: 10
- FWCI
- 17.02
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- University College Dublin
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Keywords
- exopolysaccharide production, Lactobacillus bulgaricus, chemically defined medium, carbon source, lactose, glucose, orotic acid, oxygen, pH, temperature, stationary phase, rhamnose, fermentation conditions
- MeSH terms
- Culture Media, Glucose, Hydrogen-Ion Concentration, Lactobacillus, Lactose, Monosaccharides, Polysaccharides, Bacterial
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