Evaluation of Substrata Effect on Cell Adhesion Properties Using Freestanding Poly(l-lactic acid) Nanosheets
Abstract
Investigation of the interactions between cells and material surfaces is important not only for the understanding of cell biology but also for the development of smart biomaterials. In this study, we investigated the substrate-related effects on the interaction between cell and polymeric ultrathin film (nanosheet) by modulating the mechanical properties of the nanosheet with a metal substrate or mesh. A freestanding polymeric nanosheet with tens-of-nanometers thickness composed of poly(L-lactic acid) (PLLA nanosheet) was fabricated by combination of a spin-coating technique and a water-soluble sacrificial layer. The freestanding PLLA nanosheet was collected on a stainless steel mesh (PLLA-mesh) and subsequently used for cell adhesion studies, comparing the results to the ones on a control SiO(2) substrate coated with an ultrathin layer of PLLA (PLLA-substrate). The adhesion of rat cardiomyocytes (H9c2) was evaluated on both samples after 24 h of culture. The PLLA-mesh with the tens-of-nanometers thick nanosheets induced an anisotropic adhesion of H9c2, while H9c2 on the PLLA-substrate showed an isotropic adhesion independent from the nanosheet thickness. Interestingly, an increment in the nanosheet thickness in the PLLA-mesh samples reduced the cellular anisotropy and led to a similar morphology to the PLLA-substrate. Considering the huge discrepancy of Young's modulus between PLLA nanosheet (3.5-4.2 GPa) and metal substrate (hundreds of GPa), cell adhesion was mechanically r…
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Size and shape, rather than stiffness, play a critical role in biocompatibility, based on evaluation of materials with stiffness ranging from soft alginate hydrogels (<100 kPa) to hard stainless steel (100s of GPa).
“Notably, our evaluation of a spectrum of materials with a range of mechanical stiffness properties varying from soft (Alginate hydrogels, < 100 kPa)34 to hard (Stainless steel, 100s of GPa)35 demonstrates that size and shape rather than stiffness plays a critical role in biocompatibility.”
From Size- and shape-dependent foreign body immune response to materials implanted in rodents and non-human primates (2015) · cites this paper as [35]
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Firpo, G., & Valbusa, U. (2016) · doi:10.1002/9781118831779.ch8
- Authors
- Fujie, T., Ricotti, L., Desii, A., Menciassi, A., Dario, P., & Mattoli, V.
- Affiliations
- Waseda University (JP)
- Scuola Superiore Sant'Anna (IT)
- Italian Institute of Technology (IT)
- Corresponding author
- Fujie, Toshinori
- Mattoli, Virgilio
- Venue
- Langmuir
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-09-28
- Volume
- 27
- Issue
- 21
- Pages
- 13173-13182
- ISSN
- 0743-7463 (ISSN-L)
- 1520-5827
- OpenAlex
- W2330244276
- Cited by
- 52
- References
- 45
- Citations by year
- 2024: 2 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 7 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 9 · 2015: 7 · 2014: 2 · 2013: 6 · 2012: 3
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Langmuir (publishedVersion)
- PubMed (publishedVersion)
- CINECA IRIS Institutional Research Information System (Sant'Anna School of Advanced Studies) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- cell adhesion, substrate stiffness, Young's modulus, nanosheet thickness, cardiomyocytes, H9c2 cells, stainless steel mesh substrate, spin coating, biomaterials, cell-substrate interaction
- MeSH terms
- Animals, Cell Adhesion, Metals, Polyesters, Polymers, Silicon Dioxide, Surface Properties, Lactic Acid, Myocytes, Cardiac, Nanostructures, Rats, Mechanical Phenomena
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