The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Salt-Triggered Peptide Folding and Consequent Self-Assembly into Hydrogels with Tunable Modulus
Abstract
Intramolecular folding events, triggered by the presence of salt, induce the self-assembly of β-hairpin peptides into hydrogel networks at physiological conditions. At pH 7.4 and low ionic strength solution conditions, dilute, homogeneous solutions of peptide (≤2 wt %) exhibit the viscosity of pure water. Circular dichroism spectroscopy shows that, at pH 7.4 in the absence of salt, peptides are unfolded. By raising the ionic strength of the solution, electrostatic interactions between charged amino acids within the peptide are screened, and a β-hairpin conformation is adopted. Folded β-hairpin molecules supramolecularly assemble via hydrophobic collapse and hydrogen bonding into a three-dimensional hydrogel network. FTIR and X-ray scattering data demonstrate that these hydrogels are rich in β-sheet. Dynamic oscillatory rheological measurements demonstrate that the resultant supramolecular structure forms an elastic material whose structure, and thus modulus, can be tuned by salt concentration and temperature. Storage moduli of hydrogels increase with increasing salt concentration. Robust hydrogelation is also observed in cell growth media at physiological conditions. Transmission electron microscopy reveals that the hydrogel elasticity arises from a network nanostructure consisting of semiflexible fibrillar assemblies.
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Cites
Hydrogels in pharmaceutical formulations
Peppas, N. (2000) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/s0939-6411(00)00090-4 · Cited by 4,011
Biodegradable block copolymers as injectable drug-delivery systems
Jeong, B., Bae, Y. H., Lee, D. S., & Kim, S. W. (1997) · Nature · doi:10.1038/42218 · Cited by 1,997
Volume phase transition in a nonionic gel
Hirokawa, Y., & Tanaka, T. (1984) · The Journal of Chemical Physics · doi:10.1063/1.447548 · Cited by 1,604
Collapse of Gels in an Electric Field
Tanaka, T., Nishio, I., Sun, S.-T., & Ueno-Nishio, S. (1982) · Science · doi:10.1126/science.218.4571.467 · Cited by 1,296
Peptide-amphiphile nanofibers: A versatile scaffold for the preparation of self-assembling materials
Hartgerink, J. D., Beniash, E., & Stupp, S. I. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.072699999 · Cited by 1,240
Reversible Hydrogels from Self-Assembling Artificial Proteins
Petka, W. A., Harden, J. L., McGrath, K. P., Wirtz, D., & Tirrell, D. A. (1998) · Science · doi:10.1126/science.281.5375.389 · Cited by 1,076
Thermal analysis of the volume phase transition with N-isopropylacrylamide gels
Otake, K., Inomata, H., Konno, M., & Saito, S. (1990) · Macromolecules · doi:10.1021/ma00203a049 · Cited by 742
Measurement of the β-sheet-forming propensities of amino acids
Minor, D. L., & Kim, P. S. (1994) · Nature · doi:10.1038/367660a0 · Cited by 593
Rheological characterisation of polymer gels
Kavanagh, G. M., & Ross-Murphy, S. B. (1998) · Progress in Polymer Science · doi:10.1016/s0079-6700(97)00047-6 · Cited by 581
Influence of CO2 emission rates on the stability of the thermohaline circulation
Stocker, T. F., & Schmittner, A. (1997) · Nature · doi:10.1038/42224 · Cited by 560
Aggeli, A., Bell, M., Carrick, L. M., Fishwick, C. W. G., Harding, R., Mawer, P. J., Radford, S. E., Strong, A. E., & Boden, N. (2003) · Journal of the American Chemical Society · doi:10.1021/ja021047i · Cited by 491
Pochan, D. J., Schneider, J. P., Kretsinger, J., Ozbas, B., Rajagopal, K., & Haines, L. (2003) · Journal of the American Chemical Society · doi:10.1021/ja0353154 · Cited by 463
Self-citationOsmotic Swelling of Polyacrylate Hydrogels in Physiological Salt Solutions
Horkay, F., Tasaki, I., & Basser, P. J. (2000) · Biomacromolecules · doi:10.1021/bm9905031 · Cited by 449
pH-Responsive Gels of Hydrophobically Modified Poly(acrylic acid)
Philippova, O. E., Hourdet, D., Audebert, R., & Khokhlov, A. R. (1997) · Macromolecules · doi:10.1021/ma970957v · Cited by 375
Torres-Lugo, M., & Peppas, N. A. (1999) · Macromolecules · doi:10.1021/ma990541c · Cited by 299
Caplan, M. R., Moore, P. N., Zhang, S., Kamm, R. D., & Lauffenburger, D. A. (2000) · Biomacromolecules · doi:10.1021/bm005586w · Cited by 286
Lashuel, H. A., LaBrenz, S. R., Woo, L., Serpell, L. C., & Kelly, J. W. (2000) · Journal of the American Chemical Society · doi:10.1021/ja9937831 · Cited by 285
Gestational Zinc Deprivation in Mice: Persistence of Immunodeficiency for Three Generations
Beach, R. S., Gershwin, M. E., & Hurley, L. S. (1982) · Science · doi:10.1126/science.7123244 · Cited by 219
Rheological Study on the Fractal Nature of the Protein Gel Structure
Ikeda, S., Foegeding, E. A., & Hagiwara, T. (1999) · Langmuir · doi:10.1021/la9817415 · Cited by 217
Pandit, N., Trygstad, T., Croy, S., Bohorquez, M., & Koch, C. (2000) · Journal of Colloid and Interface Science · doi:10.1006/jcis.1999.6628 · Cited by 216
A complex clathrate hydrate structure showing bimodal guest hydration
Udachin, K. A., & Ripmeester, J. A. (1999) · Nature · doi:10.1038/17097 · Cited by 149
McMillan, R. A., & Conticello, V. P. (2000) · Macromolecules · doi:10.1021/ma9921091 · Cited by 141
Calcium binding and salt-induced structural changes of native and preheated .beta.-lactoglobulin
Jeyarajah, S., & Allen, J. C. (1994) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf00037a012 · Cited by 121
Atmospheric Residence Time of CH 3 Br Estimated from the Junge Spatial Variability Relation
Colman, J. J., Blake, D. R., & Rowland, F. S. (1998) · Science · doi:10.1126/science.281.5375.392 · Cited by 95
Cited by
Stimulus-responsive hydrogels: theory, modern advances, and applications
Koetting, M. C., Peters, J. T., Steichen, S. D., & Peppas, N. A. (2015) · Materials Science and Engineering: R: Reports · doi:10.1016/j.mser.2015.04.001 · Cited by 1,191
O'Leary, L. E. R., Fallas, J. A., Bakota, E. L., Kang, M. K., & Hartgerink, J. D. (2011) · Nature Chemistry · doi:10.1038/nchem.1123 · Cited by 667
Self-assembling materials for therapeutic delivery
Branco, M. C., & Schneider, J. P. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2008.09.018 · Cited by 462
Self-citationInherent Antibacterial Activity of a Peptide-Based β-Hairpin Hydrogel
Salick, D. A., Kretsinger, J. K., Pochan, D. J., & Schneider, J. P. (2007) · Journal of the American Chemical Society · doi:10.1021/ja076300z · Cited by 351
Self-citationDong, H., Paramonov, S. E., Aulisa, L., Bakota, E. L., & Hartgerink, J. D. (2007) · Journal of the American Chemical Society · doi:10.1021/ja072536r · Cited by 344
Metadata
- Authors
- Ozbas, B., Kretsinger, J., Rajagopal, K., Schneider, J. P., & Pochan, D. J.
- Venue
- Macromolecules
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-08-25
- Volume
- 37
- Issue
- 19
- Pages
- 7331-7337
- ISSN
- 0024-9297 (ISSN-L)
- 1520-5835
- OpenAlex
- W2236781334
- Cited by
- 429
- References
- 24
- FWCI
- 5.78
- Type
- journal article
- Language
- en
- Open access
- closed
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