The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
The Use of Nanoarrays for Highly Sensitive and Selective Detection of Human Immunodeficiency Virus Type 1 in Plasma
Abstract
Arrays of antibodies with well-defined feature size and spacing are necessary for developing highly sensitive and selective immunoassays to detect macromolecules in complex solutions. Here we report the application of nanometer-scale antibody array-based analysis to determine the presence of the human immunodeficiency virus type 1 (HIV-1) in blood samples. Dip-pen nanolithography (DPN) was used to generate nanoscale patterns of antibodies against the HIV-1 p24 antigen on a gold surface. Feature sizes were less than 100-nanometers, and the activity of the antibody was preserved. HIV-1 p24 antigen in plasma obtained directly from HIV-1-infected patients was hybridized to the antibody array in situ, and the bound protein was hybridized to a gold antibody-functionalized nanoparticle probe for signal enhancement. The nanoarray features in the three-component sandwich assay were confirmed by atomic force microscopy (AFM). Demonstration of measurable amounts of HIV-1 p24 antigen in plasma obtained from men with less than 50 copies of RNA per ml of plasma (corresponding to 0.025 pg per ml) illustrates that the nanoarray-based assay can exceed the limit of detection of conventional enzyme-linked immunosorbent assay (ELISA)-based immunoassays (5 pg per ml of plasma) by more than 1000-fold.
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Citations
Cites
Global Analysis of Protein Activities Using Proteome Chips
Zhu, H., Bilgin, M., Bangham, R., Hall, D., Casamayor, A., Bertone, P., Lan, N., Jansen, R., Bidlingmaier, S., Houfek, T., et al. (2001) · Science · doi:10.1126/science.1062191 · Cited by 1,884
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Nanostructures in Biodiagnostics
Rosi, N. L., & Mirkin, C. A. (2005) · Chemical Reviews · doi:10.1021/cr030067f · Cited by 4,603
Self-citationNew Approaches to Nanofabrication: Molding, Printing, and Other Techniques
Gates, B. D., Xu, Q., Stewart, M., Ryan, D., Willson, C. G., & Whitesides, G. M. (2005) · Chemical Reviews · doi:10.1021/cr030076o · Cited by 2,020
Biswas, A., Bayer, I. S., Biris, A. S., Wang, T., Dervishi, E., & Faupel, F. (2012) · Advances in Colloid and Interface Science · doi:10.1016/j.cis.2011.11.001 · Cited by 909
Applications of dip-pen nanolithography
Salaita, K., Wang, Y., & Mirkin, C. A. (2007) · Nature Nanotechnology · doi:10.1038/nnano.2007.39 · Cited by 845
Self-citationNanotechnology, nanotoxicology, and neuroscience
Suh, W. H., Suslick, K. S., Stucky, G. D., & Suh, Y.-H. (2009) · Progress in Neurobiology · doi:10.1016/j.pneurobio.2008.09.009 · Cited by 406
Metadata
- Authors
- Lee, K.-B., Kim, E.-Y., Mirkin, C. A., & Wolinsky, S. M.
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2004-09-09
- Volume
- 4
- Issue
- 10
- Pages
- 1869-1872
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- OpenAlex
- W1987822226
- Cited by
- 241
- References
- 1
- FWCI
- 4.37
- Type
- journal article
- Language
- en
- Open access
- closed
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