Bioactive DNA-Peptide Nanotubes Enhance the Differentiation of Neural Stem Cells Into Neurons
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We report the construction of DNA nanotubes covalently functionalized with the cell adhesion peptide RGDS as a bioactive substrate for neural stem cell differentiation. Alteration of the Watson–Crick base pairing program that builds the nanostructures allowed us to probe independently the effect of nanotube architecture and peptide bioactivity on stem cell differentiation. We found that both factors instruct synergistically the preferential differentiation of the cells into neurons rather than astrocytes.
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Similar work has shown promise in stem cell differentiation and neural regeneration following spinal injury in vivo, further demonstrating the utility of peptide-based materials in cell culture.
“Similar work has shown promise in stem cell differentiation and neural regeneration following spinal injury in vivo200–202, further demonstrating the utility of peptide-based materials in cell culture.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [200]
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- Authors
- Stephanopoulos, N., Freeman, R., North, H. A., Sur, S., Jeong, S. J., Tantakitti, F., Kessler, J. A., & Stupp, S. I.
- Affiliations
- Northwestern University (US)
- Corresponding author
- Stupp, Samuel I.
- ORCID
- Venue
- Nano Letters
- Publisher
- American Chemical Society (ACS)
- Published
- 2014-12-29
- Volume
- 15
- Issue
- 1
- Pages
- 603-609
- ISSN
- 1530-6984 (ISSN-L)
- 1530-6992
- PMC
- PMC4296924
- OpenAlex
- W2287978221
- Cited by
- 138
- References
- 65
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 5 · 2023: 10 · 2022: 17 · 2021: 14 · 2020: 12 · 2019: 18 · 2018: 21 · 2017: 14 · 2016: 10 · 2015: 8
- FWCI
- 4.38
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Nano Letters (publishedVersion, other-oa)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Neurological Disorders and Stroke (1F32NS077728-01A1)
- National Institute of Biomedical Imaging and Bioengineering (5RO1EB003806-09)
- European Molecular Biology Organization (ALTF 233-2012)
- Craig H. Neilsen Foundation (NA)
- Royal Thai Government (NA)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced biosensing and bioanalysis techniques
- Physical Sciences › Materials Science › Biomaterials › Supramolecular Self-Assembly in Materials
- Physical Sciences › Engineering › Biomedical Engineering › Nanotechnology research and applications
- Keywords
- DNA nanotubes, RGDS peptide, neural stem cells, neuronal differentiation, astrocyte differentiation, covalent functionalization, Watson-Crick base pairing, cell adhesion peptides
- MeSH terms
- Animals, Cell Differentiation, Cells, Cultured, DNA, Neurons, Oligopeptides, Nanotubes, Peptide, Mice, Neural Stem Cells
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