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Temperature Sensing Using Fluorescent Nanothermometers

Open access
Vetrone, Fiorenzo, Naccache, Rafik, Zamarrón, Alicia, Juarranz de la Fuente, Angeles, Sanz-Rodríguez, Francisco, Martinez Maestro, Laura, Martín Rodriguez, Emma, Jaque, Daniel, García Solé, José, Capobianco, John A.·2010·ACS Nano

Abstract

Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF(4):Er(3+),Yb(3+) nanoparticles, where the intensity ratio of the green fluorescence bands of the Er(3+) dopant ions ((2)H(11/2) --> (4)I(15/2) and (4)S(3/2) --> (4)I(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF(4):Er(3+),Yb(3+) nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.

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Authors
Vetrone, F., Naccache, R., Zamarrón, A., Juarranz de la Fuente, A., Sanz-Rodríguez, F., Martinez Maestro, L., Martín Rodriguez, E., Jaque, D., García Solé, J., & Capobianco, J. A.
Venue
ACS Nano
Published
2010-05-04
Volume
4
Issue
6
Pages
3254-3258
ISSN
  • 1936-0851 (ISSN-L)
  • 1936-086X
OpenAlex
W2030395871
Cited by
1,505
References
0
FWCI
22.39
Type
journal article
Language
en
Open access
green

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